Anc1与通用转录因子TFIID和TFIIF的催化亚基、染色质重塑复合物RSC和INO80以及组蛋白乙酰转移酶复合物NuA3相互作用。

Anc1 interacts with the catalytic subunits of the general transcription factors TFIID and TFIIF, the chromatin remodeling complexes RSC and INO80, and the histone acetyltransferase complex NuA3.

作者信息

Kabani Mehdi, Michot Katia, Boschiero Claire, Werner Michel

机构信息

Laboratoire de Physiogénomique, Service de Biochimie et Génétique Moléculaire, Departement de Biologie Joliot Curie, Bâtiment 144, CEA/Saclay, F-91191 Gif-sur-Yvette, France.

出版信息

Biochem Biophys Res Commun. 2005 Jul 1;332(2):398-403. doi: 10.1016/j.bbrc.2005.04.158.

DOI:10.1016/j.bbrc.2005.04.158

PMID:15896708

Abstract

The Anc1 protein co-purifies with general transcription factors, chromatin remodeling complexes, and histone modification enzymes and is required for efficient transcription in yeast. We show here that Anc1 interacts with Tsm1, Tfg1, Sth1, Ino80, and Sas3 that are, respectively, the catalytic subunits of the general transcription factors TFIID and TFIIF, of the chromatin remodeling complexes RSC and INO80, and of the histone H3-acetyltransferase complex NuA3. We show that Anc1 is required for growth on galactose as the sole carbon source, and that it is recruited to the UAS of the GAL1 gene after induction.

摘要

Anc1蛋白与通用转录因子、染色质重塑复合物和组蛋白修饰酶共同纯化，是酵母高效转录所必需的。我们在此表明，Anc1与Tsm1、Tfg1、Sth1、Ino80和Sas3相互作用，它们分别是通用转录因子TFIID和TFIIF、染色质重塑复合物RSC和INO80以及组蛋白H3-乙酰转移酶复合物NuA3的催化亚基。我们表明，Anc1是在以半乳糖作为唯一碳源时生长所必需的，并且在诱导后它被招募到GAL1基因的上游激活序列（UAS）。