Reikerås O, Ytrehus K
Department of Orthopaedic Surgery, University of Tromsø, Norway.
Scand J Clin Lab Invest. 1992 Apr;52(2):113-8. doi: 10.3109/00365519209088774.
In this study the protective effects of removing oxygen free radicals during ischaemia and reperfusion of skeletal muscle were investigated. The bilateral gracilis muscle model was used in six dogs. Both muscles were made ischaemic for 4 h, followed by reperfusion for 60 min. To remove oxygen free radicals, superoxide dismutase and catalase were given 10 min before ischaemia and during the first 30 min of reperfusion in one muscle; the other muscle served as a control. Muscle blood flow was recorded during the first 35 min of reperfusion. At the end of reperfusion the contents of high-energy phosphates and glycogen were measured. Furthermore, the metabolic burst of leukocytes (chemiluminescence) was determined. Flow as well as creatine phosphate were always highest in the treated muscles as compared with the control muscles (p less than 0.05). Adenosine triphosphate and glycogen were highest in all but one case. Spontaneous leukocyte chemiluminescence was significantly reduced in venous blood from the control muscle and insignificantly reduced in blood from the treated muscle as compared with blood from the aorta. The results are indirect evidence that reactive oxygen metabolites play a role in the genesis of ischaemia-reperfusion injury of skeletal muscle and that treatment with scavenger enzymes may have protective effects.
本研究探讨了在骨骼肌缺血再灌注过程中清除氧自由基的保护作用。选用6只犬，采用双侧股薄肌模型。双侧肌肉均缺血4小时，然后再灌注60分钟。为清除氧自由基，在一侧肌肉缺血前10分钟及再灌注的前30分钟给予超氧化物歧化酶和过氧化氢酶；另一侧肌肉作为对照。在再灌注的前35分钟记录肌肉血流量。再灌注结束时，测定高能磷酸盐和糖原的含量。此外，还测定了白细胞的代谢爆发（化学发光）。与对照肌肉相比，处理过的肌肉血流量和磷酸肌酸含量总是最高（p<0.05）。除1例情况外，三磷酸腺苷和糖原含量均最高。与主动脉血相比，对照肌肉静脉血中自发白细胞化学发光明显降低，处理过的肌肉血中自发白细胞化学发光降低不明显。结果间接证明活性氧代谢产物在骨骼肌缺血再灌注损伤的发生中起作用，清除酶治疗可能具有保护作用。
