Omoe Katsuhiko, Hu Dong-Liang, Takahashi-Omoe Hiromi, Nakane Akio, Shinagawa Kunihiro
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka, Iwate 020-8550, Japan.
FEMS Microbiol Lett. 2005 May 15;246(2):191-8. doi: 10.1016/j.femsle.2005.04.007.
We describe a comprehensive detection system for 18 kinds of classical and newly described staphylococcal superantigenic toxin genes using four sets of multiplex PCR. Superantigenic toxin genotyping of Staphylococcus aureus for 69 food poisoning isolates and 97 healthy human nasal swab isolates revealed 32 superantigenic toxin genotypes and showed that many S. aureus isolates harbored multiple toxin genes. Analysis of the relationship between toxin genotypes and toxin genes encoding profiles of mobile genetic elements suggests its possible role in determining superantigenic toxin genotypes in S. aureus as combinations of toxin gene-encoding mobile genetic elements.
我们描述了一种使用四组多重PCR对18种经典和新描述的葡萄球菌超抗原毒素基因进行全面检测的系统。对69株食物中毒分离株和97株健康人鼻拭子分离株的金黄色葡萄球菌超抗原毒素基因分型显示出32种超抗原毒素基因型，并表明许多金黄色葡萄球菌分离株携带多个毒素基因。对毒素基因型与编码移动遗传元件的毒素基因图谱之间关系的分析表明，作为编码毒素基因的移动遗传元件的组合，其在确定金黄色葡萄球菌超抗原毒素基因型中可能发挥作用。
