Hibma M, Griffin J F
Microbiology Department University of Otago, Dunedin, New Zealand.
Vet Immunol Immunopathol. 1992 Mar;31(3-4):267-78. doi: 10.1016/0165-2427(92)90014-h.
Monoclonal antibodies (mAb) which react with cervine immunoglobulin (Ig) light chain, IgM and IgG were produced using conventional cell fusion technology. Hybridoma supernatants were initially screened for specificity against cervine Ig using an enzyme-linked immunosorbent assay (ELISA). The specificity of supernatants against size-fractionated cervine Ig was further determined. Supernatants were characterised using western blotting and autoradiographic techniques. The mAb OU1G, OU2G and OU3G were specific for cervine gamma-chain of IgG, whereas OU1L was specific for light chain of Ig. A further mAb (OU1M) bound IgM and not IgG. These mAb were found to have varying cross-reactivity against Ig from other species.
利用传统细胞融合技术制备了与鹿免疫球蛋白（Ig）轻链、IgM和IgG发生反应的单克隆抗体（mAb）。最初使用酶联免疫吸附测定（ELISA）针对鹿Ig对杂交瘤上清液进行特异性筛选。进一步确定上清液对大小分级的鹿Ig的特异性。使用蛋白质印迹法和放射自显影技术对上清液进行表征。单克隆抗体OU1G、OU2G和OU3G对IgG的鹿γ链具有特异性，而OU1L对Ig的轻链具有特异性。另一种单克隆抗体（OU1M）与IgM结合而不与IgG结合。发现这些单克隆抗体对来自其他物种的Ig具有不同程度的交叉反应性。
