Vehring Reinhard
Nektar Therapeutics, San Carlos, California 94070, USA.
Appl Spectrosc. 2005 Mar;59(3):286-92. doi: 10.1366/0003702053585318.
Dispersive Raman spectroscopy with excitation by a red diode laser is suitable for quantitative crystallinity measurements in powders for pulmonary drug delivery. In spray-dried mixtures of salmon calcitonin and mannitol, all three crystalline polymorphs of mannitol and amorphous mannitol were unambiguously identified and their mass fractions were measured with a limit of quantification of about 5%. The instrument design offered high sensitivity and adequate background suppression, resulting in a low limit of detection in the range of 0.01% to 1%. This spectroscopy method has significant advantages over established techniques regarding specificity, sensitivity, and sample requirements.
采用红色二极管激光器激发的色散拉曼光谱适用于肺部药物递送用粉末的结晶度定量测量。在鲑鱼降钙素和甘露醇的喷雾干燥混合物中，甘露醇的三种结晶多晶型物和无定形甘露醇均被明确鉴定，其质量分数的测量定量限约为5%。该仪器设计具有高灵敏度和足够的背景抑制能力，检测下限低至0.01%至1%。与现有技术相比，这种光谱方法在特异性、灵敏度和样品要求方面具有显著优势。
