Martinez Aurora Esmeralda Traverso, Silverio Karina Gonzales, Fogo Jose Carlos, Kirkwood Keith Lough, Rossa Carlos
Department of Diagnosis and Surgery, School of Dentistry of Araraquara, State University of Sao Paulo, Rua Humaita 1680, 14801-903 Araraquara, Sao Paulo, Brazil.
Clin Oral Investig. 2005 Sep;9(3):180-6. doi: 10.1007/s00784-004-0294-z. Epub 2005 May 19.
The purpose of study was to evaluate fibroblast attachment and cellular morphology on root surfaces chemically conditioned with nicotine or cotinine. A secondary objective was to determine if mechanical scaling and root planning of these chemically conditioned surfaces would alter cellular attachment. Root surface dentin specimens were prepared from uniradicular teeth of non-smoking patients. Specimens were randomly assigned to two experimental groups: no treatment (chemical conditioning only) and scaling and root planning after conditioning (SRPC). The concentrations of the tested substances were in the range of 0-1 mg/mL (nicotine) and 0-1 g/mL (cotinine). After a 24-h conditioning period, dentin slices were incubated with continuous lineage of fibroblastic cells from rat (McCoy cells) for another 24 h. Specimens were prepared for SEM analysis and microphotographs. The statistical analysis of the data indicated significant alteration of cellular morphology on fibroblasts that were grown on root surface exposed to nicotine concentrations greater than 1 ?g/mL. This effect of nicotine was not reduced by SRPC. On the other hand, in the SRPC group cellular density was greater. For cotinine-conditioned specimens, the greater concentrations also led to alteration on morphology, and these alterations were observed in the SRPC group as well. Cotinine did not induce significant changes on cellular density. The results indicated that fibroblasts are negatively influenced by nicotine present on the dentin substrate and also that scaling may reduce these effects. Cotinine treatment on root surfaces may alter cell morphology and density but these effects were less severe than that promoted by nicotine, and were not affected by scaling.
本研究的目的是评估用尼古丁或可替宁进行化学预处理的牙根表面上的成纤维细胞附着情况和细胞形态。第二个目的是确定对这些经化学预处理的表面进行机械刮治和根面平整是否会改变细胞附着。牙根表面牙本质标本取自非吸烟患者的单根牙。标本被随机分为两个实验组：不进行处理（仅化学预处理）和预处理后进行刮治和根面平整（SRPC）。受试物质的浓度范围为0 - 1 mg/mL（尼古丁）和0 - 1 g/mL（可替宁）。经过24小时的预处理期后，将牙本质切片与来自大鼠的成纤维细胞连续谱系（McCoy细胞）再孵育24小时。制备标本用于扫描电子显微镜（SEM）分析并拍摄显微照片。数据的统计分析表明，在暴露于浓度大于1μg/mL尼古丁的牙根表面生长的成纤维细胞，其细胞形态发生了显著改变。SRPC并未减轻尼古丁的这种作用。另一方面，在SRPC组中细胞密度更高。对于用可替宁预处理的标本，较高浓度也导致了形态改变，并且在SRPC组中也观察到了这些改变。可替宁未引起细胞密度的显著变化。结果表明，牙本质基质上存在的尼古丁对成纤维细胞有负面影响，并且刮治可能会减轻这些影响。牙根表面用可替宁处理可能会改变细胞形态和密度，但这些影响不如尼古丁引起的严重，并且不受刮治的影响。
