Sheikh Kashif, Förster Jochen, Nielsen Lars K
Department of Chemical Engineering, The University of Queensland, St. Lucia, 4072 QLD, Australia, and Fluxome Sciences A/S, Søltofts Plads, Building 223, 2800 Lyngby, Denmark.
Biotechnol Prog. 2005 Jan-Feb;21(1):112-21. doi: 10.1021/bp0498138.
The reconstructed cellular metabolic network of Mus musculus, based on annotated genomic data, pathway databases, and currently available biochemical and physiological information, is presented. Although incomplete, it represents the first attempt to collect and characterize the metabolic network of a mammalian cell on the basis of genomic data. The reaction network is generic in nature and attempts to capture the carbon, energy, and nitrogen metabolism of the cell. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, including transport reactions between the compartments and the extracellular medium. The reaction list consists of 872 internal metabolites involved in a total of 1220 reactions, whereof 473 relate to known open reading frames. Initial in silico analysis of the reconstructed model is presented.
本文展示了基于注释基因组数据、通路数据库以及当前可用的生化和生理信息重建的小家鼠细胞代谢网络。尽管并不完整，但这是首次尝试基于基因组数据收集和表征哺乳动物细胞的代谢网络。该反应网络本质上是通用的，旨在捕捉细胞的碳、能量和氮代谢。代谢反应被划分在细胞质和线粒体之间，包括各区间与细胞外介质之间的转运反应。反应列表包含872种内部代谢物，涉及总共1220个反应，其中473个与已知的开放阅读框相关。文中还展示了对重建模型的初步计算机模拟分析。
