Weber Wilfried, Malphettes Laetitia, Rinderknecht Matthias, Schoenmakers Ronald G, Spielmann Manuela, Keller Bettina, van de Wetering Petra, Weber Cornelia C, Fussenegger Martin
Institute of Chemical- and Bio-Engineering, Swiss Federal Institute of Technology, ETH Hoenggerberg HCI F115, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
Biotechnol Prog. 2005 Jan-Feb;21(1):178-85. doi: 10.1021/bp0498995.
Technologies for regulated expression of multiple transgenes in mammalian cells have gathered momentum for bioengineering, gene therapy, drug discovery, and gene-function analyses. Capitalizing on recently developed mammalian transgene modalities (QuoRex) derived from Streptomyces coelicolor, we have designed a flexible and highly compatible expression vector set that enables desired transgene/siRNA control in response to the nontoxic butyrolactone SCB1. The construction-kit-like expression portfolio includes (i) multicistronic (pTRIDENT), (ii) autoregulated, (iii) bidirectional (pBiRex), (iv) oncoretro- and lentiviral transduction, and (v) RNA polymerase II-based siRNA transcription-fine-tuning vectors for straightforward implementation of QuoRex-controlled (trans)gene modulation in mammalian cells.
用于在哺乳动物细胞中调控多个转基因表达的技术在生物工程、基因治疗、药物发现和基因功能分析等领域发展迅猛。利用最近从天蓝色链霉菌衍生而来的哺乳动物转基因模式（QuoRex），我们设计了一套灵活且高度兼容的表达载体，能够根据无毒的丁内酯SCB1实现对所需转基因/siRNA的控制。这种类似构建试剂盒的表达组合包括：（i）多顺反子（pTRIDENT），（ii）自动调控型，（iii）双向（pBiRex），（iv）致癌逆转录病毒和慢病毒转导，以及（v）基于RNA聚合酶II的siRNA转录微调载体，用于在哺乳动物细胞中直接实现QuoRex控制的（转）基因调控。
