Amini M, Hamedani M Pirali, Vosooghi M, Nabavi M, Shafiee A
Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Anal Bioanal Chem. 2005 Jul;382(5):1265-8. doi: 10.1007/s00216-005-3249-6. Epub 2005 May 20.
An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10-500 ng ml(-1).
