Sasaki N, Kaido M, Okuno T, Mise K
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Arch Virol. 2005 Jun;150(6):1231-40. doi: 10.1007/s00705-004-0481-x. Epub 2005 Jan 27.
The movement protein (MP) of Brome mosaic virus (BMV) depends on the coat protein (CP) to mediate the cell-to-cell movement of BMV and CCMV(B3a), a recombinant Cowpea chlorotic mottle virus (CCMV) expressing BMV MP. Previous studies identified gain-of-function mutations in the central region of BMV MP that enable CCMV(B3a) to adapt to a resistant host. This study demonstrates that all adaptation-related MPs can partially or almost fully mediate the cell-to-cell movement of CCMV(B3a) and BMV without CP. Based on these results, we discuss adaptation mechanisms of CCMV(B3a) and the role of the central region of MP in the determination of virus movement mode.
雀麦花叶病毒（BMV）的运动蛋白（MP）依赖外壳蛋白（CP）来介导BMV以及CCMV（B3a）（一种表达BMV MP的重组豇豆花叶病毒（CCMV））在细胞间的移动。先前的研究在BMV MP的中央区域鉴定出功能获得性突变，这些突变使CCMV（B3a）能够适应抗性宿主。本研究表明，所有与适应性相关的MP都能部分或几乎完全介导CCMV（B3a）和无CP的BMV在细胞间的移动。基于这些结果，我们讨论了CCMV（B3a）的适应机制以及MP中央区域在病毒移动模式确定中的作用。
