Zhao Chengtian, Qi Jingjing, Meng Anming
State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Dev Dyn. 2005 Jul;233(3):1157-62. doi: 10.1002/dvdy.20438.
Members of the ATF/CREB (activating transcription factor/cAMP-responsive element binding protein) transcription factor family play diverse roles in controlling cell proliferation, apoptosis, and oncogenesis, as well as in embryonic development of vertebrates. We identified two zebrafish orthologs of human ATF7 gene: atf7a and atf7b. Whole-mount in situ hybridization shows that zebrafish atf7a is first expressed in the notochord precursors at 80% epiboly stage and then in the developing notochord during segmentation. The expression of atf7a is positively regulated by ntl, flh, and spr2, which are involved in development of the notochord. In contrast, atf7b is maternally expressed and during embryogenesis its mRNA is ubiquitously distributed, showing an expression pattern similar to that of mammalian Atf7.
ATF/CREB（激活转录因子/环磷酸腺苷反应元件结合蛋白）转录因子家族成员在控制细胞增殖、凋亡和肿瘤发生以及脊椎动物胚胎发育中发挥着多种作用。我们鉴定出了人类ATF7基因的两个斑马鱼直系同源基因：atf7a和atf7b。整体原位杂交显示，斑马鱼atf7a在卵裂球80%外包期时首先在脊索前体中表达，然后在体节形成期间在发育中的脊索中表达。atf7a的表达受到ntl、flh和spr2的正向调控，这些基因参与脊索的发育。相比之下，atf7b是母源表达的，在胚胎发育过程中其mRNA广泛分布，显示出与哺乳动物Atf7相似的表达模式。
