von Hofsten J, Larsson A, Olsson P E
Department of Molecular Biology, Umeå University, Umeå, Sweden.
Dev Dyn. 2005 Jun;233(2):595-604. doi: 10.1002/dvdy.20335.
ff1d is a novel zebrafish FTZ-F1 gene with sequence characteristics indicating similar basic regulatory mechanisms as the previously characterized ff1 based on the presence of an FTZ-F1 box in the DNA binding domain and an interactive domain (I-Box) and an AF-2 in the ligand binding domain. The highest sequence similarity was found between ff1d and ff1b (NR5A4), a gene previously shown to be a functional homolog to the steroidogenic factor 1 (SF-1). The expression pattern of ff1d was comparable to ff1b both in brain and gonads in adults and in the pituitary and interrenal cells in embryos. SF-1 is crucial in mammalian steroidogenesis and in sex determination by regulating the anti-Mullerian hormone (AMH). In fish, AMH has not been described previously. In this study, we cloned a partial zebrafish AMH. AMH was detected in growing oocytes, the ovarian follicular layer and testicular Sertoli cells, similar to the mammalian pattern, suggesting a conserved role between zebrafish and mammalian AMH. Teleosts lack a gene homolog to SRY, which constitute the universal testis-determining factor in mammalian sex determination. Comparison of sequences and expression patterns indicate that ff1d is a new candidate for sex determination and differentiation in a way similar to SF-1, possibly involving AMH.
ff1d是一种新型斑马鱼FTZ-F1基因，其序列特征表明，基于DNA结合域中存在FTZ-F1框、一个相互作用域（I-Box）以及配体结合域中的AF-2，其基本调控机制与先前鉴定的ff1相似。ff1d与ff1b（NR5A4）的序列相似性最高，ff1b是一个先前已被证明是类固醇生成因子1（SF-1）功能同源物的基因。ff1d在成年斑马鱼的脑和性腺以及胚胎的垂体和肾间细胞中的表达模式与ff1b相当。SF-1通过调节抗苗勒管激素（AMH）在哺乳动物类固醇生成和性别决定中起关键作用。在鱼类中，此前尚未描述过AMH。在本研究中，我们克隆了部分斑马鱼AMH。在生长中的卵母细胞、卵巢滤泡层和睾丸支持细胞中检测到了AMH，这与哺乳动物的模式相似，表明斑马鱼和哺乳动物的AMH具有保守作用。硬骨鱼缺乏与SRY同源的基因，SRY是哺乳动物性别决定中普遍的睾丸决定因子。序列和表达模式的比较表明，ff1d是性别决定和分化的一个新候选基因，其方式类似于SF-1，可能涉及AMH。
