Tejero F, Finol H J
Section of Parasitology, Institute of Tropical Zoology, Faculty of Sciences, Central University of Venezuela, Caracas, Venezuela.
J Submicrosc Cytol Pathol. 2004 Jul-Oct;36(3-4):313-7.
Axenic cultures of pure Trypanosoma rangeli were used to investigate the relation between ultrastructure and activity in the mitochondrion. Every other day, ultrathin sections were obtained from cultivated flagellates and, subsequently, observed in order to register changes in the cytoarchitecture of the organelle. Culture samples were incubated in tetrazolium salts to determine the mitochondrion's metabolic state. The results show a high correspondence between mitochondrion ultrastructural shape and function of the same organelle in populations of T. rangeli maintained under in vitro conditions.
利用纯净的克氏锥虫无菌培养物来研究线粒体超微结构与活性之间的关系。每隔一天，从培养的鞭毛虫中获取超薄切片，随后进行观察，以记录该细胞器细胞结构的变化。将培养样品在四唑盐中孵育，以确定线粒体的代谢状态。结果表明，在体外条件下培养的克氏锥虫群体中，线粒体的超微结构形状与该细胞器的功能之间存在高度相关性。
