Qin Guo, Xicheng Wang
Department of Biological Science and Biotechnology, School of Life Sciences and Engineering, Tsinghua University, Beijing, China.
Protein Pept Lett. 2005 May;12(4):369-73. doi: 10.2174/0929866053765699.
Thermal inactivation and unfolding of the dimeric arginine kinase (AK) from sea cucumber Stichopus japonicus was investigated. The activation energy was calculated to be 388 kJ/mol. Based on the analysis of the denaturation course at 58 degrees C, a model is suggested for the thermal unfolding of this dimeric AK. In addition, the effect of free Mg(2+) and the potential biological significance on the thermal unfolding of dimeric AK is discussed.
研究了日本刺参二聚体精氨酸激酶（AK）的热失活和去折叠过程。计算得到的活化能为388 kJ/mol。基于对58℃下变性过程的分析，提出了该二聚体AK热去折叠的模型。此外，还讨论了游离Mg(2+)的影响以及其对二聚体AK热去折叠的潜在生物学意义。
