茶多酚（-）-表没食子儿茶素没食子酸酯对宫颈腺癌细胞系生长的抑制作用。
Inhibitory effect of the tea polyphenol, (-)-epigallocatechin gallate, on growth of cervical adenocarcinoma cell lines.
作者信息
Noguchi Mitsuyo, Yokoyama Masatoshi, Watanabe Sumiko, Uchiyama Michiko, Nakao Yoshifumi, Hara Kouichi, Iwasaka Tsuyoshi
机构信息
Department of Obstetrics and Gynecology, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
出版信息
Cancer Lett. 2006 Mar 28;234(2):135-42. doi: 10.1016/j.canlet.2005.03.053.
To investigate the effect of (-)-epigallocatechin gallate (EGCG) on cervical adenocarcinoma, we performed a cell proliferation assay. TRAP assay is used for telomerase activity, flow cytometry analysis and pKi-67 immunofluoroscein staining in cervical adenocarcinoma cell lines (OMC-4, TMCC-1). Our results showed that EGCG inhibited the proliferation assay, TRAP assay for telomerase activity of both cell lines. Although cell apoptosis was induced, we observed that the expression of pKi-67 was suppressed. Our data suggest that EGCG may be effective for the treatment of cervical adenocarcinoma. The mechanisms of the anti-tumor effects were revealed to be the inhibition of telomerase activity, the induction of apoptosis and cell cycle dysregulation.
为研究（-）-表没食子儿茶素没食子酸酯（EGCG）对宫颈腺癌的影响，我们进行了细胞增殖试验。采用端粒重复序列扩增法（TRAP法）检测宫颈腺癌细胞系（OMC-4、TMCC-1）的端粒酶活性，并进行流式细胞术分析和pKi-67免疫荧光染色。我们的结果表明，EGCG抑制了这两种细胞系的增殖试验及TRAP法检测的端粒酶活性。虽然诱导了细胞凋亡，但我们观察到pKi-67的表达受到抑制。我们的数据表明，EGCG可能对宫颈腺癌的治疗有效。抗肿瘤作用机制显示为抑制端粒酶活性、诱导细胞凋亡和细胞周期失调。
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