海胆发育过程中DNA甲基化模式的变化。

Fronk J, Tank G A, Langmore J P

Biophysics Research Division, University of Michigan, Ann Arbor 48109.

Biochem J. 1992 May 1;283 ( Pt 3)(Pt 3):751-3. doi: 10.1042/bj2830751.

Cytosine methylation of developmentally regulated genes of the sea urchin Strongylocentrotus purpuratus was studied by using restriction-endonuclease digestion and Southern blotting. The single-copy bindin gene, the family of five cytoplasmic actin genes and the 400-fold-repeated set of five early histone genes were mostly unmethylated, but some sites exhibited partial methylation that varied throughout development. This shows that in echinoderms the methylation of DNA is not confined to the non-transcribed portion of the genome, as previously believed [Bird, Tagart & Smith (1979) Cell 17, 889-901], and may play a role in transcriptional regulation.

通过使用限制性内切酶消化和Southern印迹法，对紫海胆（Strongylocentrotus purpuratus）发育调控基因的胞嘧啶甲基化进行了研究。单拷贝的结合蛋白基因、五个细胞质肌动蛋白基因家族以及由五个早期组蛋白基因组成的400倍重复序列大多未甲基化，但有些位点表现出部分甲基化，且在整个发育过程中有所变化。这表明在棘皮动物中，DNA甲基化并不局限于基因组的非转录部分，这与之前的观点[Bird, Tagart & Smith (1979) Cell 17, 889 - 901]不同，并且可能在转录调控中发挥作用。