Rosales M J, Cordón G Peréz, Moreno M Sánchez, Sánchez C Marín
Departamento de Parasitología, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, España.
Acta Trop. 2005 Jul;95(1):74-8. doi: 10.1016/j.actatropica.2005.03.009.
The present study confirms the existence of extracellular stages of Cryptosporidiumparvum during in vitro culture on MDCK, HCT 8 and Vero cells as well as alveolar macrophages, by optic, Nomarski and transmission electron microscopy images. Extracellular trophozoite/gamont, stages in syzygy, zygotes and spores with eight sporozoites were seen in the supernatant of the cultures. The first ultrastructural images of extracellular stages of C. parvum are shown in this study. The morphology of these stages, which have characteristics similar to those of some gregarines, support the contention that Cryptosporidium has closer affinity with gregarines. It also supports the necessity of reconsidering the life cycle of Cryptosporidium and the classification within the coccidia.
本研究通过光学显微镜、诺马斯基显微镜和透射电子显微镜图像，证实了微小隐孢子虫在MDCK、HCT 8和Vero细胞以及肺泡巨噬细胞体外培养过程中存在细胞外阶段。在培养上清液中可见细胞外滋养体/配子体、配对阶段、合子和带有八个子孢子的孢子。本研究展示了微小隐孢子虫细胞外阶段的首张超微结构图像。这些阶段的形态与某些簇虫相似，支持了隐孢子虫与簇虫有更密切亲缘关系的观点。这也支持了重新考虑隐孢子虫生命周期和球虫纲内分类的必要性。
