两种G12家族G蛋白，Gα12和Gα13，表现出不同的亚细胞定位。

Two G12 family G proteins, G alpha12 and G alpha13, show different subcellular localization.

作者信息

Yamazaki Junya, Katoh Hironori, Yamaguchi Yoshiaki, Negishi Manabu

机构信息

Laboratory of Molecular Neurobiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

出版信息

Biochem Biophys Res Commun. 2005 Jul 8;332(3):782-6. doi: 10.1016/j.bbrc.2005.05.023.

DOI:10.1016/j.bbrc.2005.05.023

PMID:15907792

Abstract

The G alpha subunits of the G12 family of heterotrimeric G proteins, G alpha12 and G alpha13, are closely related in sequences and some effectors, but they often act through different pathways or bind to different proteins. We have examined subcellular distribution of these two G proteins and found that endogenous G alpha12 and G alpha13 localize in membrane and cytoplasmic fractions, respectively. Exogenously expressed G alpha12 and G alpha13 also localize in membrane and cytoplasmic fractions, respectively, in COS-7 cells. Stimulation of lysophosphatidic acid receptor coupled to G alpha13 markedly promotes the translocation of G alpha13 from cytoplasm to membrane. This different localization of G alpha12 and G alpha13 may explain some of the nonoverlapping actions of G alpha12 and G alpha13.

摘要

异源三聚体G蛋白G12家族的Gα亚基Gα12和Gα13在序列和一些效应器方面密切相关，但它们通常通过不同的途径起作用或与不同的蛋白质结合。我们研究了这两种G蛋白的亚细胞分布，发现内源性Gα12和Gα13分别定位于膜和细胞质组分中。在COS-7细胞中，外源性表达的Gα12和Gα13也分别定位于膜和细胞质组分中。与Gα13偶联的溶血磷脂酸受体的刺激显著促进Gα13从细胞质向膜的转位。Gα12和Gα13的这种不同定位可能解释了Gα12和Gα13的一些非重叠作用。