Chistoserdov A Y, Boyd J, Mathews F S, Lidstrom M E
W.M. Keck Laboratories 138-78, California Institute of Technology, Pasadena 91125.
Biochem Biophys Res Commun. 1992 May 15;184(3):1181-9. doi: 10.1016/s0006-291x(05)80007-5.
The mau gene cluster from Paracoccus denitrificans was cloned. The regions of a cloned fragment carrying genes for the small and the large subunit of the methylamine dehydrogenase were identified and sequenced. Open reading frames for the MADH small subunit gene and the MADH large subunit gene were identified. Three other open reading frames coding polypeptides with unknown function were found in the sequence. The small subunit gene sequence data reveal that the MADH small subunit polypeptide from P. denitrificans has an unusual leader sequence and contains the tryptophan tryptophyl quinone cofactor. The MADH small subunit genes and the parts of the open reading frames found upstream of them in the genome of M. extorquens AM1 and P. denitrificans have considerable similarity. The sequence data have been used for refinement of the X-ray crystallographic structure of the MADH from P. denitrificans, and key conserved residues have been identified.
对反硝化副球菌的mau基因簇进行了克隆。鉴定并测序了携带甲胺脱氢酶小亚基和大亚基基因的克隆片段区域。确定了MADH小亚基基因和MADH大亚基基因的开放阅读框。在该序列中发现了另外三个编码功能未知多肽的开放阅读框。小亚基基因序列数据表明，反硝化副球菌的MADH小亚基多肽具有异常的前导序列，并含有色氨酸-色氨酸醌辅因子。在扭脱甲基杆菌AM1和反硝化副球菌的基因组中，MADH小亚基基因及其上游发现的开放阅读框部分具有相当大的相似性。这些序列数据已用于改进反硝化副球菌MADH的X射线晶体结构，并确定了关键的保守残基。
