Zebrower M, Beeber C, Kieras F J
NYS Institute for Basic Research in Developmental Disabilities, Staten Island 10314.
Biochem Biophys Res Commun. 1992 May 15;184(3):1293-300. doi: 10.1016/s0006-291x(05)80023-3.
Skin fibroblasts lines established from patients with Alzheimer's disease and old normal individuals were cultured with 35S-sodium sulfate and 3H-glucosamine. Proteoglycans were isolated and characterized. Sulfate incorporation into proteoglycans increased in Alzheimer's disease fibroblasts relative to normal controls. These increases changed the ratio of chondroitin sulfate to heparan sulfate proteoglycan from 1.4 to 1.7 (p = 0.0012) and decreased the ratio of cell to medium proteoglycans from 0.32 to 0.26 in normal and Alzheimer fibroblasts (p = 0.006), respectively. HPLC analysis of the disaccharides produced by chondroitinase ABC revealed no differences in composition between proteoglycans of Alzheimer and normal fibroblasts in either the cell or medium fraction. However, analysis of disaccharides produced by heparinase plus heparitinase showed differences in composition in the medium but not the cell fraction. delta UA-GlcNS was increased by 30% while delta UA-GlcNS-6S was reduced by 40% in Alzheimer's disease.
从阿尔茨海默病患者和老年正常个体中建立的皮肤成纤维细胞系，用35S - 硫酸钠和3H - 葡糖胺进行培养。分离并鉴定蛋白聚糖。与正常对照相比，阿尔茨海默病成纤维细胞中蛋白聚糖的硫酸盐掺入增加。这些增加使硫酸软骨素与硫酸乙酰肝素蛋白聚糖的比例从1.4变为1.7（p = 0.0012），并使正常和阿尔茨海默病成纤维细胞中细胞与培养基中蛋白聚糖的比例分别从0.32降至0.26（p = 0.006）。用软骨素酶ABC产生的二糖进行的HPLC分析显示，在细胞或培养基部分中，阿尔茨海默病和正常成纤维细胞的蛋白聚糖之间在组成上没有差异。然而，用肝素酶加乙酰肝素酶产生的二糖分析显示，在培养基部分组成有差异，但在细胞部分没有。在阿尔茨海默病中，δUA - GlcNS增加了30%，而δUA - GlcNS - 6S减少了40%。
