蛋白激酶C（PKC）在骨吸收中的作用：特异性PKC抑制剂1-烷基-2-甲基甘油的作用

Role of protein kinase C (PKC) in bone resorption: effect of the specific PKC inhibitor 1-alkyl-2-methylglycerol.

作者信息

Bos M P, Most W, van Leeuwen J P, Herrmann-Erlee M P

机构信息

Laboratory of Cell Biology and Histology, University of Leiden, Rijnsburgerweg, The Netherlands.

出版信息

Biochem Biophys Res Commun. 1992 May 15;184(3):1317-23. doi: 10.1016/s0006-291x(05)80026-9.

DOI:10.1016/s0006-291x(05)80026-9

PMID:1590794

Abstract

The specific inhibitor of protein kinase C, 1-O-alkyl-2-O-methylglycerol (AMG), was studied for its effect on bone resorption, measured as 45Ca-release, in fetal mouse calvariae. AMG (1 to 50 microM) had no effect on basal bone resorption. AMG inhibited parathyroid hormone (40 nM) induced bone resorption in a dose-dependent manner. Resorption induced by 1,25 (OH)2-vitamin D3 (10 nM) or prostaglandin E2 (5 microM) was also inhibited by AMG. The release of beta-glucuronidase activity paralleled the course of the 45Ca-release. The production of interleukin 6, induced by parathyroid hormone, in fetal rat calvarial osteoblasts was not affected by AMG. AMG (1 to 50 microM) had no cytotoxic effects on cells or calvariae. From these results it is concluded that protein kinase C may have an important role in the regulation of bone resorption.

摘要

研究了蛋白激酶C的特异性抑制剂1-O-烷基-2-O-甲基甘油（AMG）对胎鼠颅骨骨吸收的影响，通过测量45Ca释放来评估。AMG（1至50微摩尔）对基础骨吸收无影响。AMG以剂量依赖的方式抑制甲状旁腺激素（40纳摩尔）诱导的骨吸收。1,25（OH）2-维生素D3（10纳摩尔）或前列腺素E2（5微摩尔）诱导的骨吸收也被AMG抑制。β-葡萄糖醛酸酶活性的释放与45Ca释放过程平行。甲状旁腺激素诱导的胎鼠颅骨成骨细胞中白细胞介素6的产生不受AMG影响。AMG（1至50微摩尔）对细胞或颅骨无细胞毒性作用。从这些结果可以得出结论，蛋白激酶C可能在骨吸收调节中起重要作用。