Pearson K G, Acharya H, Fouad K
Department of Physiology, University of Alberta, Edmonton, Alta., Canada T6G 2H7.
J Neurosci Methods. 2005 Oct 15;148(1):36-42. doi: 10.1016/j.jneumeth.2005.04.006.
With the increasing use of normal and genetically modified mice in the field of motor physiology, there is a need for a simple and reliable technique for recording electromyographic (EMG) activity in behaving mice. Here, we describe a new method for the fabrication and implantation of fine EMG recording electrodes into multiple muscles of adult mice. This method minimizes surgical damage to the muscles and the connecting leads have only a modest influence on leg movements when electrodes are implanted into distal muscles. We demonstrate that excellent EMG recordings can be obtained during walking, swimming and scratching for the vastus lateralis, tibialis anterior and gastrocnemius muscles in normal adult mice. EMG recordings were also made in a mutant EphA4 mouse to demonstrate the utility of the method for examining motor patterns in genetically modified animals. We also developed a method for constructing highly reflective markers that could be viewed over a range of orientations to measure the kinematics of legs movements during stepping. A commercial motion analysis system was used to track six markers during walking and to synchronize video and EMG data during walking sequences.
随着正常小鼠和基因改造小鼠在运动生理学领域的使用日益增加，需要一种简单可靠的技术来记录行为小鼠的肌电图（EMG）活动。在此，我们描述了一种将精细EMG记录电极制作并植入成年小鼠多块肌肉的新方法。该方法将对肌肉的手术损伤降至最低，并且当电极植入远端肌肉时，连接导线对腿部运动的影响较小。我们证明，在正常成年小鼠的行走、游泳和抓挠过程中，可从股外侧肌、胫骨前肌和腓肠肌获得出色的EMG记录。我们还在突变型EphA4小鼠中进行了EMG记录，以证明该方法在检查基因改造动物运动模式方面的实用性。我们还开发了一种构建高反射标记的方法，这些标记可以在一系列方向上被观察到，以测量行走过程中腿部运动的运动学。在行走过程中，使用商业运动分析系统跟踪六个标记，并在行走序列中同步视频和EMG数据。
