Hlavacek Otakar, Bourens Myriam, Salone Veronique, Lachacinski Nicole, Lemaire Claire, Dujardin Geneviève
Centre de Génétique Moléculaire, Avenue de la Terrasse, 91198-Gif sur Yvette, France.
Gene. 2005 Jul 18;354:53-7. doi: 10.1016/j.gene.2005.03.016.
Oxa1p is a key component of the machinery for the insertion of membrane proteins in mitochondria, and in the yeast Saccharomyces cerevisiae, the deletion of OXA1 impairs the biogenesis of the three respiratory complexes of dual genetic origin. Oxa1p is formed from three domains located in the intermembrane space, the inner membrane and the mitochondrial matrix. We have isolated a high copy suppressor able to partially compensate for the respiratory deficiency caused by a large deletion of the matrix domain. We show that the suppressor gene corresponds to the nuclear transcriptional activator Hap4p which is known to regulate respiratory functions.
Oxa1p是线粒体中膜蛋白插入机制的关键组成部分，在酿酒酵母中，OXA1的缺失会损害双重遗传起源的三种呼吸复合体的生物合成。Oxa1p由位于膜间隙、内膜和线粒体基质中的三个结构域组成。我们分离出了一个高拷贝抑制子，它能够部分补偿由基质结构域的大片段缺失所导致的呼吸缺陷。我们发现该抑制子基因对应于已知调控呼吸功能的核转录激活因子Hap4p。
