Takara E A, Pasini-Cabello S D, Cerutti S, Gásquez J A, Martinez L D
Department of Analytical Chemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Chacabuco and Pedernera, P.O. Box 375, 5700 San Luis, Argentina.
J Pharm Biomed Anal. 2005 Sep 15;39(3-4):735-9. doi: 10.1016/j.jpba.2005.04.010.
A method for the on-line preconcentration of copper using a minicolumn packed with activated carbon and its subsequent determination by inductively coupled plasma optical emission spectrometry (ICP-OES) coupled with flow injection (FI) was studied. In order to determinate the copper concentration present in parenteral solutions; it was retained on activated carbon (AC) at pH 9.5. A sensitivity enrichment factor of 30-fold was obtained with respect to the copper determination by ICP-OES without preconcentration. The detection limit for the preconcentration of 25 ml of sample was 0.1 microgl(-1). The precision for the ten replicate determinations at the 2.5 microgl(-1) Cu level was 3.0% relative standard deviation (R.S.D.), calculated with the peak heights. The calibration graph using the preconcentration method for cooper species was linear with a correlation coefficient of 0.9996 at levels near the detection limits up to at least 200 microgl(-1). The method was successfully applied to the determination of copper in parenteral solutions.
研究了一种使用填充活性炭的微型柱在线预富集铜，并随后通过电感耦合等离子体发射光谱法（ICP - OES）结合流动注射（FI）进行测定的方法。为了测定肠胃外营养液中存在的铜浓度，在pH 9.5条件下将其保留在活性炭（AC）上。相对于未进行预富集的ICP - OES法测定铜，获得了30倍的灵敏度富集因子。对25 ml样品进行预富集的检测限为0.1 μg l⁻¹。在2.5 μg l⁻¹铜水平下进行十次重复测定的精密度为相对标准偏差（R.S.D.）3.0%，以峰高计算。使用预富集方法测定铜物种的校准曲线在接近检测限直至至少200 μg l⁻¹的水平范围内呈线性，相关系数为0.9996。该方法成功应用于肠胃外营养液中铜的测定。
