Lobachevska O, Kyjak N, Khorkavtsiv O, Dovgalyuk A, Kit N, Klyuchivska O, Stoika R, Ripetsky R, Cove D
Institute of Ecology of the Carpathians, National Academy of Sciences of Ukraine, 11 Stefanyk Street, 79000 Lviv, Ukraine.
Cell Biol Int. 2005 Mar;29(3):181-6. doi: 10.1016/j.cellbi.2005.02.001.
Epigenetically-determined apogamy in aposporous regenerants of the moss Pottia intermedia persists during vegetative propagation, the capacity of apogamy being inherited by individual aposporous protonemal cells. To test Bauer-Lazarenko's proposal that stable apogamy in mosses may be due to some self-replicating cytoplasmic factor, the effects of different metabolic stress treatments on the expression of apogamy have been tested. Chronic metabolic stress caused by long-term growth of autotrophic aposporous protonema on mineral medium with 0.25% of casamino acids and on Murashiga-Skoog (MS) medium with sucrose and phytohormones, as well as by transitory action of high kinetin concentration, have a much stronger influence on the expression of apogamy, than short-term stress treatments with RNase and Pb(2+). Apogamy has been found to be lost stably, after prolonged growth on MS medium containing kinetin and ABA. The proposal that the capacity for apogamy is related to the release of aposporous protonemal cells from a putative factor for apogamy is discussed.
中间立碗藓无孢子生殖再生体中由表观遗传决定的无融合生殖在营养繁殖过程中持续存在，无融合生殖能力由单个无孢子原丝体细胞遗传。为了验证鲍尔 - 拉扎连科提出的苔藓中稳定的无融合生殖可能归因于某种自我复制的细胞质因子这一观点，已测试了不同代谢应激处理对无融合生殖表达的影响。自养型无孢子原丝体在含有0.25%酪蛋白氨基酸的矿物培养基上以及在含有蔗糖和植物激素的Murashiga - Skoog（MS）培养基上长期生长所导致的慢性代谢应激，以及高浓度激动素的短暂作用，对无融合生殖表达的影响比用核糖核酸酶和Pb(2+)进行的短期应激处理要强得多。已发现，在含有激动素和脱落酸的MS培养基上长期生长后，无融合生殖会稳定丧失。本文讨论了无融合生殖能力与无孢子原丝体细胞从假定的无融合生殖因子中释放出来有关这一观点。
