Goldmann Wolfgang H, Cantiello Horacio F, Chasan Bernard
Zentralinstitut für Biomedizinische Technik, Friedrich-Alexander-Universität, Erlangen-Nürnberg, D-91052 Erlangen, Germany.
Cell Biol Int. 2005 Mar;29(3):245-8. doi: 10.1016/j.cellbi.2004.12.003.
Myosin II controls the viscoelastic behavior of actin filaments, interacting with actin in an energy-dependent manner. Replacing adenosine triphosphate with adenosine diphosphate changes actomyosin sliding to cross-linking. Rheological measurements show a 3-4-fold increase of the elastic portion G' in actin filaments when myosin II is present at a molar ratio r(MA)=1:200. This observation is supported by the demonstration of inactive myosin heads along actin filaments using atomic force microscopy.
肌球蛋白II以能量依赖的方式与肌动蛋白相互作用，控制着肌动蛋白丝的粘弹性行为。用二磷酸腺苷取代三磷酸腺苷会使肌动球蛋白的滑动转变为交联。流变学测量表明，当肌球蛋白II以摩尔比r(MA)=1:200存在时，肌动蛋白丝中弹性部分G'增加3至4倍。使用原子力显微镜对沿肌动蛋白丝的无活性肌球蛋白头部进行的演示支持了这一观察结果。
