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Combining suppression subtractive hybridization and microarrays to map the intraspecies phylogeny of Flavobacterium psychrophilum.结合抑制消减杂交和微阵列技术绘制嗜冷黄杆菌种内系统发育图谱。
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2
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本文引用的文献

1
Flavobacterium psychrophilum in rainbow trout, Oncorhynchus mykiss (Walbaum), hatcheries: studies on broodstock, eggs, fry and environment.虹鳟（Oncorhynchus mykiss (Walbaum)）孵化场中的嗜冷黄杆菌：亲鱼、鱼卵、鱼苗及环境研究
J Fish Dis. 2005 Jan;28(1):39-47. doi: 10.1111/j.1365-2761.2004.00598.x.
2
Selective discrimination of Listeria monocytogenes epidemic strains by a mixed-genome DNA microarray compared to discrimination by pulsed-field gel electrophoresis, ribotyping, and multilocus sequence typing.与脉冲场凝胶电泳、核糖体分型和多位点序列分型相比，利用混合基因组DNA微阵列对单核细胞增生李斯特菌流行菌株进行选择性鉴别。
J Clin Microbiol. 2004 Nov;42(11):5270-6. doi: 10.1128/JCM.42.11.5270-5276.2004.
3
Simultaneous discrimination between 15 fish pathogens by using 16S ribosomal DNA PCR and DNA microarrays.通过16S核糖体DNA聚合酶链式反应（PCR）和DNA微阵列同时鉴别15种鱼类病原体。
Appl Environ Microbiol. 2004 Jul;70(7):4216-21. doi: 10.1128/AEM.70.7.4216-4221.2004.
4
Protective immunity in rainbow trout Oncorhynchus mykiss following immunization with distinct molecular mass fractions isolated from Flavobacterium psychrophilum.用从嗜冷黄杆菌中分离出的不同分子量组分免疫虹鳟（Oncorhynchus mykiss）后的保护性免疫
Dis Aquat Organ. 2004 Apr 21;59(1):17-26. doi: 10.3354/dao059017.
5
Development of genetic techniques for the psychrotrophic fish pathogen Flavobacterium psychrophilum.嗜冷鱼类病原菌嗜冷黄杆菌的遗传技术开发。
Appl Environ Microbiol. 2004 Jan;70(1):581-7. doi: 10.1128/AEM.70.1.581-587.2004.
6
Flavobacterium psychrophilum infections in salmonid fish.鲑科鱼类的嗜冷黄杆菌感染
J Fish Dis. 2003 Oct;26(10):563-74. doi: 10.1046/j.1365-2761.2003.00488.x.
7
Passive immunization of rainbow trout, Oncorhynchus mykiss (Walbaum), against Flavobacterium psychrophilum, the causative agent of bacterial coldwater disease and rainbow trout fry syndrome.虹鳟（Oncorhynchus mykiss，Walbaum）针对嗜冷黄杆菌（细菌性冷水病和虹鳟鱼苗综合征的病原体）的被动免疫。
J Fish Dis. 2003 Jul;26(7):371-84. doi: 10.1046/j.1365-2761.2003.00468.x.
8
Purification and characterization of a membrane glycoprotein from the fish pathogen Flavobacterium psychrophilum.
J Appl Microbiol. 2003;94(6):1120-7. doi: 10.1046/j.1365-2672.2003.01946.x.
9
Mixed-genome microarrays reveal multiple serotype and lineage-specific differences among strains of Listeria monocytogenes.混合基因组微阵列揭示了单核细胞增生李斯特菌菌株之间多种血清型和谱系特异性差异。
J Clin Microbiol. 2003 Feb;41(2):632-9. doi: 10.1128/JCM.41.2.632-639.2003.
10
Discrimination among Listeria monocytogenes isolates using a mixed genome DNA microarray.使用混合基因组DNA微阵列对单核细胞增生李斯特菌分离株进行鉴别。
Vet Microbiol. 2003 Apr 29;92(4):351-62. doi: 10.1016/s0378-1135(02)00423-6.

结合抑制消减杂交和微阵列技术绘制嗜冷黄杆菌种内系统发育图谱。

Combining suppression subtractive hybridization and microarrays to map the intraspecies phylogeny of Flavobacterium psychrophilum.

作者信息

Soule Marilyn, Cain Kenneth, LaFrentz Stacey, Call Douglas R

机构信息

Department of Veterinary Microbiology and Pathology, Washington State University, 402 Bustad Hall, Pullman, WA 99164-7040, USA.

出版信息

Infect Immun. 2005 Jun;73(6):3799-802. doi: 10.1128/IAI.73.6.3799-3802.2005.

DOI:10.1128/IAI.73.6.3799-3802.2005
PMID:15908416
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1111865/
Abstract

Reciprocal subtractive libraries were prepared for two strains of Flavobacterium psychrophilum, one virulent and the other avirulent in a trout challenge model. Unique clones were sequenced and their distribution assessed among 34 strains. The analysis showed that F. psychrophilum is composed of two genetic lineages, possibly reflecting host specificity.

摘要

针对嗜冷黄杆菌的两个菌株构建了消减文库，其中一个菌株在鳟鱼攻毒模型中具有致病性，另一个则无致病性。对独特的克隆进行测序，并评估它们在34个菌株中的分布情况。分析表明，嗜冷黄杆菌由两个遗传谱系组成，这可能反映了宿主特异性。