Le Roy Florence, Salehzada Tamim, Bisbal Catherine, Dougherty Joseph P, Peltz Stuart W
Department of Molecular Genetics, Microbiology & Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
Nat Struct Mol Biol. 2005 Jun;12(6):505-12. doi: 10.1038/nsmb944. Epub 2005 May 22.
The antiviral and antiproliferative effects of interferons are mediated in part by the 2'-5' oligoadenylate-RNase L RNA decay pathway. RNase L is an endoribonuclease that requires 2'-5' oligoadenylates to cleave single-stranded RNA. In this report we present evidence demonstrating a role for RNase L in translation. We identify and characterize the human translation termination factor eRF3/GSPT1 as an interacting partner of RNase L. We show that interaction of eRF3 with RNase L leads to both increased translation readthrough efficiency at premature termination codons and increased +1 frameshift efficiency at the antizyme +1 frameshift site. On the basis of our results, we present a model describing how RNase L is involved in regulating gene expression by modulating the translation termination process.
干扰素的抗病毒和抗增殖作用部分是由2'-5'寡聚腺苷酸-RNase L RNA降解途径介导的。RNase L是一种核酸内切酶，需要2'-5'寡聚腺苷酸来切割单链RNA。在本报告中，我们提供了证据证明RNase L在翻译中发挥作用。我们鉴定并表征了人类翻译终止因子eRF3/GSPT1是RNase L的相互作用伴侣。我们表明，eRF3与RNase L的相互作用导致在提前终止密码子处的翻译通读效率增加，以及在抗酶+1移码位点处的+1移码效率增加。基于我们的结果，我们提出了一个模型，描述了RNase L如何通过调节翻译终止过程参与基因表达的调控。
