1α,25-二羟基维生素D3通过白细胞介素-12调节小鼠对肺炎球菌3型荚膜多糖的抗体反应。

1alpha,25-Dihydroxyvitamin D3 modulates the murine antibody response to pneumococcal capsular polysaccharide serotype 3 through IL-12.

作者信息

Jeurissen Axel, Van Etten Evelyne, Overbergh Lut, Wuyts Greet, Heremans Hubertine, Matthys Patrick, Ceuppens Jan L, Mathieu Chantal, Bossuyt Xavier

机构信息

Experimental Laboratory Medicine, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium.

出版信息

Eur J Immunol. 2005 Jun;35(6):1841-8. doi: 10.1002/eji.200425784.

DOI:10.1002/eji.200425784

PMID:15909307

Abstract

1alpha,25-Dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)] is a steroid hormone that regulates calcium metabolism. Besides, 1alpha,25(OH)(2)D(3 )also has pronounced immunomodulatory effects: it strongly inhibits dendritic cell (DC) maturation and impairs IL-12 production. We studied the effect of 1alpha,25(OH)(2)D(3 )on the antibody response to pneumococcal capsular polysaccharide (caps-PS) serotype 3. 1alpha,25(OH)(2)D(3) inhibited the IgG2a antibody response to caps-PS serotype 3. Besides, 1alpha,25(OH)(2)D(3) also inhibited IL-12 production and maturation of DC. Anti-IL-12 and exogenous IL-12, respectively, inhibited and stimulated the IgG2a antibody response to caps-PS serotype 3. Exogenous IL-12 abrogated the effect of 1alpha,25(OH)(2)D(3) on the IgG2a antibody response to caps-PS serotype 3, indicating that the effect of 1alpha,25(OH)(2)D(3) on the IgG2a antibody response to caps-PS serotype 3 was mediated through IL-12. In conclusion, we demonstrate that 1alpha,25(OH)(2)D(3) has an inhibitory effect on the IgG2a antibody response to caps-PS serotype 3, and that this effect was mediated trough IL-12.

摘要

1α,25-二羟基维生素D(3)[1α,25(OH)₂D(3)]是一种调节钙代谢的类固醇激素。此外，1α,25(OH)₂D(3)还具有显著的免疫调节作用：它强烈抑制树突状细胞(DC)成熟并损害IL-12的产生。我们研究了1α,25(OH)₂D(3)对肺炎球菌荚膜多糖(caps-PS)3型抗体反应的影响。1α,25(OH)₂D(3)抑制了对caps-PS 3型的IgG2a抗体反应。此外，1α,25(OH)₂D(3)还抑制了DC的IL-12产生和成熟。抗IL-12和外源性IL-12分别抑制和刺激了对caps-PS 3型的IgG2a抗体反应。外源性IL-12消除了1α,25(OH)₂D(3)对caps-PS 3型IgG2a抗体反应的影响，表明1α,25(OH)₂D(3)对caps-PS 3型IgG2a抗体反应的影响是通过IL-12介导的。总之，我们证明1α,25(OH)₂D(3)对caps-PS 3型的IgG2a抗体反应具有抑制作用，且这种作用是通过IL-12介导产生的。