硅胶整体膜作为分离介质。不同膜用于高效液相色谱分离的可加和性质。

Silica monolithic membrane as separation medium. Summable property of different membranes for high-performance liquid chromatographic separation.

作者信息

Hosoya Ken, Ogata Tomoyuki, Watabe Yoshiyuki, Kubo Takuya, Ikegami Tohru, Tanaka Nobuo, Minakuchi Hiroyoshi, Nakanishi Kazuki

机构信息

Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.

出版信息

J Chromatogr A. 2005 May 6;1073(1-2):123-6. doi: 10.1016/j.chroma.2004.09.042.

DOI:10.1016/j.chroma.2004.09.042

PMID:15909513

Abstract

We studied an applicability of a silica monolithic membrane as separation medium for high-performance liquid chromatography (HPLC). We prepared porous monolithic silica membranes having a three-dimensional network structure to cut and shape into a membrane separation medium. We evaluated chromatographic properties of a variety of solutes using a column containing the membranes with HPLC to elucidate summable property of the membrane separation media. In addition, we made brief study on separation of HbA1c in whole blood with the stacked" membranes having different surface characteristics in one column, which is a membrane column. We confirmed that the membrane column was able to separate HbA1c from other matrix in whole blood to some extent, and it also had an excellent ability for hydrophobic and ion exchange adsorption.

摘要

我们研究了硅胶整体柱膜作为高效液相色谱（HPLC）分离介质的适用性。我们制备了具有三维网络结构的多孔整体硅胶膜，将其切割并成型为膜分离介质。我们使用装有该膜的色谱柱通过HPLC评估了各种溶质的色谱性能，以阐明膜分离介质的可加和特性。此外，我们还对在同一根柱（即膜柱）中使用具有不同表面特性的“堆叠”膜对全血中的糖化血红蛋白（HbA1c）进行分离做了简要研究。我们证实该膜柱能够在一定程度上从全血中的其他基质中分离出HbA1c，并且它还具有出色的疏水和离子交换吸附能力。