Adewale B, Mafe M A, Oyerinde J P O
Public Health Division, Nigerian Institute of Medical Research, P. M. B. 2013, Yaba, Lagos, Nigeria.
West Afr J Med. 2005 Jan-Mar;24(1):21-5. doi: 10.4314/wajm.v24i1.28157.
Annual mass treatment with ivermectin for 12-15 years in endemic communities is the control strategy adopted by the African Programme for Onchocerciasis Control (APOC) for the control of onchocerciasis in Nigeria. This long-term treatment necessitates the use of Polymerase Chain Reaction (PCR) for the proper identification of the Onchocerca species and strains in endemic areas and also for monitoring recrudescence of infection in areas where infection has been controlled. This study, which forms part of a larger study on transmission of onchocerciasis identifies the Onchocerca volvulus strain in Ondo state using the Polymerase Chain Reaction (PCR) technique. Deoxyribonucleic acid (DNA) was extracted from the adult worm of Onchocerca parasite using the glass bead method of extraction. The repeated sequence family present in the genome of the parasite designated as 0-150bp was amplified by the polymerase chain reaction (PCR). The amplified parasites produced significant products visible as bands in a 2% agarose gel stained with ethidium bromide. Hybridization of the PCR products with specific DNA probe identified the products as forest strain of Onchocerca volvulus. The epidemiological implication of this is that there would be more of the skin lesions and low blindness rate in the area.
在流行社区每年使用伊维菌素进行12至15年的群体治疗，是非洲盘尾丝虫病控制计划（APOC）在尼日利亚采用的控制盘尾丝虫病的策略。这种长期治疗需要使用聚合酶链反应（PCR）来准确识别流行地区的盘尾丝虫种类和菌株，同时也用于监测已控制感染地区的感染复发情况。本研究是关于盘尾丝虫病传播的一项更大规模研究的一部分，采用聚合酶链反应（PCR）技术鉴定了翁多州的盘尾丝虫菌株。使用玻璃珠提取法从盘尾丝虫寄生虫的成虫中提取脱氧核糖核酸（DNA）。通过聚合酶链反应（PCR）扩增寄生虫基因组中存在的重复序列家族，该家族被指定为0 - 150bp。扩增后的寄生虫产生了在溴化乙锭染色的2%琼脂糖凝胶中可见的明显条带。PCR产物与特定DNA探针的杂交将产物鉴定为盘尾丝虫的森林菌株。这一结果的流行病学意义在于该地区将会出现更多的皮肤病变且失明率较低。
