Nadel B, Drapier A M, Andrade L, Cazenave P A, Sanchez P
Départment d'Immunologie, Institut Pasteur, Paris, France.
Int Immunol. 1992 Apr;4(4):501-7. doi: 10.1093/intimm/4.4.501.
To gain insights into the composition of the B cell repertoire, we have investigated VH gene family expression associated with individual light chains. For this purpose, we have examined the use of 12 VH gene families in a large collection of hybridomas expressing one of the four lambda light chains [lambda 1 (V1J1), lambda 2 (V2J2 and V x J2) and lambda 3 (V1J3)]. Our results show that the distribution of the VH families is very different from one lambda subtype to another. This suggests that a few substitutions between VL regions are sufficient to generate very different associated repertoires by strong selection mechanisms. Moreover, we assume that the global VH expression pattern is not random but rather composed of many preferential VH/VL associations.
为深入了解B细胞受体库的组成，我们研究了与单个轻链相关的VH基因家族表达。为此，我们在大量表达四种λ轻链之一（λ1（V1J1）、λ2（V2J2和VxJ2）以及λ3（V1J3））的杂交瘤中检测了12个VH基因家族的使用情况。我们的结果表明，VH家族的分布在不同的λ亚型之间差异很大。这表明VL区域之间的少数替换足以通过强大的选择机制产生非常不同的相关受体库。此外，我们假设整体VH表达模式并非随机，而是由许多优先的VH/VL关联组成。
