Foster J A, Herr J C
Department of Anatomy and Cell Biology, University of Virginia, Charlottesville 22908.
Biol Reprod. 1992 May;46(5):981-90. doi: 10.1095/biolreprod46.5.981.
The interaction of the human acrosomal protein SP-10 with the acrosomal membranes was analyzed by the ability of Triton X-114 (TX-114) and other agents to release SP-10 from the acrosome. Treatment of human sperm with TX-114 revealed a pool of SP-10 that was released by TX-114 and a pool of SP-10 that was TX-114-resistant. TX-114-resistant SP-10 was associated with the equatorial segment and with TX-114-resistant portions of the acrosomal matrix and the inner acrosomal membrane. Phase partitioning of TX-114-released and TX-114-resistant SP-10 pools showed that both were hydrophilic, indicating that these pools consist of proteins that are peripherally associated with, rather than integral to, the acrosomal membranes. Sequential treatments of human sperm with various agents showed that repeated washes with TX-114 or 1.5 M NaCl had little or no effect on TX-114-resistant SP-10, whereas treatment with a chaotropic salt (150 mM sodium thiocyanate) and buffers at pH extremes (pH 2.0 and 10.0) completely released this pool of SP-10 from the acrosome. Together the results suggest that SP-10 is a hydrophilic peripheral acrosomal membrane protein that may be associated with a TX-114-resistant "anchor."
通过Triton X-114（TX-114）和其他试剂从顶体释放SP-10的能力，分析了人类顶体蛋白SP-10与顶体膜的相互作用。用TX-114处理人类精子后发现，有一部分SP-10可被TX-114释放，还有一部分SP-10对TX-114具有抗性。对TX-114具有抗性的SP-10与赤道段以及顶体基质和顶体内膜中对TX-114具有抗性的部分相关联。对TX-114释放的和对TX-114具有抗性的SP-10组分进行相分离，结果表明二者均为亲水性，这表明这些组分由与顶体膜外周相关而非整合于顶体膜的蛋白质组成。用各种试剂对人类精子进行顺序处理表明，用TX-114或1.5 M NaCl反复洗涤对TX-114抗性的SP-10影响很小或没有影响，而用离液盐（150 mM硫氰酸钠）和极端pH值（pH 2.0和10.0）的缓冲液处理可使这部分SP-10从顶体中完全释放出来。这些结果共同表明，SP-10是一种亲水性外周顶体膜蛋白，可能与一种对TX-114具有抗性的“锚”相关联。
