靶向人细胞周期蛋白T1的短发夹RNA对HIV-1复制的特异性抑制作用且不诱导细胞凋亡。
Specific inhibition of HIV-1 replication by short hairpin RNAs targeting human cyclin T1 without inducing apoptosis.
作者信息
Li Zhaoyang, Xiong Yong, Peng Yu, Pan Ji'an, Chen Yu, Wu Xiaoyun, Hussain Snawar, Tien Po, Guo Deyin
机构信息
The Modern Virology Research Centre and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
出版信息
FEBS Lett. 2005 Jun 6;579(14):3100-6. doi: 10.1016/j.febslet.2005.04.074.
RNA interference (RNAi), a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), can be used not only as a research tool but also as a therapeutic strategy for viral infection. We demonstrated that intracellular expression of short hairpin RNA (shRNA) targeting human cyclin T1 (hCycT1), a cellular factor essential for transcription of messenger and genomic RNAs from the long terminal repeat promoter of provirus of human immunodeficiency virus type 1 (HIV-1), could effectively suppress the replication of HIV-1. We also showed that downregulation of hCycT1 did not cause apoptotic cell death, therefore, targeting cellular factor hCycT1 by shRNAs may provide an attractive approach for genetic therapy of HIV-1 infection in the future.
RNA干扰（RNAi）是一种由小干扰RNA（siRNA）介导的序列特异性RNA降解机制，不仅可作为一种研究工具，还可作为一种针对病毒感染的治疗策略。我们证明，针对人细胞周期蛋白T1（hCycT1）的短发夹RNA（shRNA）在细胞内表达，hCycT1是从人类免疫缺陷病毒1型（HIV-1）前病毒的长末端重复启动子转录信使RNA和基因组RNA所必需的一种细胞因子，它能够有效抑制HIV-1的复制。我们还表明，hCycT1的下调不会导致凋亡性细胞死亡，因此，通过shRNA靶向细胞因子hCycT1可能为未来HIV-1感染的基因治疗提供一种有吸引力的方法。
Zotero 插件