Gielen Alexander W, Lobell Anna, Lidman Olle, Khademi Mohsen, Olsson Tomas, Piehl Fredrik
Department of Clinical Neuroscience, Neuroimmunology Unit, CMM, L8:04, Karolinska University Hospital, Karolinska Institutet, SE 171 76, Stockholm, Sweden.
J Neuroimmunol. 2005 Jul;164(1-2):93-104. doi: 10.1016/j.jneuroim.2005.04.004.
Expression of T cell immunoglobulin- and mucin-domain-containing molecules (TIMs) can be used as T helper (Th) differentiation markers in the human and mouse. We examined the expression of TIM-1 and -3 mRNAs in rat MBP(63-88)-induced experimental autoimmune encephalomyelitis (EAE). TIM-3 expression was upregulated in the spinal cord during EAE and following antigen restimulation of the encephalitogenic TCRBV8S2+ population. Interestingly, TIM-3 expression was also detected by in situ hybridization in resident cells of the nervous system. TIM-1 was expressed in B cells but not in resident CNS cells and TIM-1 mRNA levels in spinal cord were unchanged throughout the course of EAE. These results support the notion that TIM-3 can also be used as a Th1 differentiation marker in the rat. However, expression of TIM-1 and -3 is not restricted solely to T cells and the presence of TIM-3 in resident CNS cells may indicate a role for this molecule in the interaction between the nervous and immune systems.
含T细胞免疫球蛋白和粘蛋白结构域分子（TIMs）的表达可作为人和小鼠中辅助性T细胞（Th）分化的标志物。我们检测了大鼠髓鞘碱性蛋白（MBP）（63-88）诱导的实验性自身免疫性脑脊髓炎（EAE）中TIM-1和-3 mRNA的表达。在EAE期间以及对致脑炎的TCRBV8S2+群体进行抗原再刺激后，脊髓中TIM-3的表达上调。有趣的是，通过原位杂交在神经系统的驻留细胞中也检测到了TIM-3的表达。TIM-1在B细胞中表达，但在中枢神经系统驻留细胞中不表达，并且在EAE的整个病程中脊髓中TIM-1 mRNA水平没有变化。这些结果支持TIM-3也可作为大鼠中Th1分化标志物的观点。然而，TIM-1和-3的表达并不局限于T细胞，并且驻留中枢神经系统细胞中TIM-3的存在可能表明该分子在神经和免疫系统之间的相互作用中发挥作用。
