Tang Shunming, Zhao Qiaoling, Yi Yongzhu, Zhang Zhifang, Li Yiren
Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang.
Biosci Biotechnol Biochem. 2005 May;69(5):1014-7. doi: 10.1271/bbb.69.1014.
The heat shock cognate 70-4 protein gene promoter (HSC70-4p) from Bombyx mori (BmHSC70-4p) is an ideal candidate for the transgenic silkworm due to its high transcriptional activity, and the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) functions as an enhancer for several promoters. Using luciferase as a reporter gene and transient expression system in vivo and in vitro, we found that BmNPVhr3 can significantly increase the transcriptional activity of BmHSC70-4p and HSC70-4p from Bombyx mandarina (BmandHSC70-4p). Moreover, the transcriptional activity of the combination of BmHSC70-4p and BmNPVhr3 changed with developmental stages and hormone titers. These results suggest that the combination of BmHSC70-4 promoter and BmNPVhr3 enhancer is more effective candidate for the transgene or stable cell expression system in Bombyx mori.
家蚕热休克同源蛋白70-4基因启动子（BmHSC70-4p）因其高转录活性，是转基因家蚕的理想候选者，而家蚕核型多角体病毒同源区域3（BmNPVhr3）可作为多种启动子的增强子。利用荧光素酶作为报告基因以及体内和体外瞬时表达系统，我们发现BmNPVhr3可显著提高BmHSC70-4p以及野桑蚕热休克同源蛋白70-4基因启动子（BmandHSC70-4p）的转录活性。此外，BmHSC70-4p与BmNPVhr3组合的转录活性随发育阶段和激素滴度而变化。这些结果表明，BmHSC70-4启动子与BmNPVhr3增强子的组合是家蚕转基因或稳定细胞表达系统更有效的候选者。
Zotero 插件