Fidente Paola, Di Giovanni Carmen, Seccia Serenella, Morrica Patrizia
Dipartimento di Chimica Farmaceutica e Tossicologica, Università degli Studi di Napoli Federico II, via D. Montesano 49, 80131 Napoli, Italy.
Biomed Chromatogr. 2005 Dec;19(10):766-70. doi: 10.1002/bmc.513.
We describe a method for determination of cymoxanil, 1-2-cyano-2-methoxy(iminoacetyl)-3-ethylurea, in drinking water and in soil, using reversed-phase HPLC with UV detection at 240 nm and a mobile phase of acetonitrile-water (30:70, v/v). Fortified water samples (1.0 L) were extracted with solid-phase extraction on Strata X. Soil samples (20 g) were extracted with acetone and the extracts were transferred onto Strata C18E. The recoveries of cymoxanil from water and soil samples were over 85% for each fortification level. The RDS were within the range 1.7-4.1% for water and 0.9-1.2% for soil samples. After optimization of the extraction and separation conditions, the method was validated.
我们描述了一种使用反相高效液相色谱法，在240nm波长下进行紫外检测，以乙腈 - 水（30:70，v/v）作为流动相，测定饮用水和土壤中霜脲氰（1-2-氰基-2-甲氧基（亚氨基乙酰基）-3-乙基脲）的方法。加标水样（1.0L）通过在Strata X上进行固相萃取来提取。土壤样品（20g）用丙酮提取，提取物转移到Strata C18E上。对于每个加标水平，霜脲氰在水样和土壤样品中的回收率均超过85%。相对标准偏差（RSD）对于水样在1.7 - 4.1%范围内，对于土壤样品在0.9 - 1.2%范围内。在优化提取和分离条件后，该方法得到了验证。
