Kemapunmanus Marisa, Sretrirutchai Somporn, Khuntikij Paiwon, Pradutkanchana Sukone, Pradutkanchana Jintana
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Southeast Asian J Trop Med Public Health. 2004 Dec;35(4):863-7.
This was a prospective evaluation of four immunodiagnostic assays for human leptospirosis, including the indirect immunofluorescence test (IFA), the microscopic agglutination test (MAT), the LEPTO dipstick, and the latex agglutination (LA) test. Four hundred ninety-two serum samples were collected from 348 patients who presented with acute fever without localizing signs. The sensitivities of the IFA, MAT, Dipstick, and LA were 91.9, 76.6, 77.4, and 83.1%, respectively. The specificities of these assays were 100.0, 100.0, 89.3, and 83.5, respectively. Even though IFA showed the highest overall sensitivity and specificity, when acute sera were considered, the LA was the most sensitive (28.7%). All 3 genus specific antibody assays had broad reactivity against various serogroups. The MAT is best suited for the reference laboratory, where it can be maintained with the battery of live antigens; the IFA is suited for a laboratory with sophisticated equipment and technical expertise; the Dipstick and LA are suitable for peripheral laboratories which lack expensive equipment and expertise.
这是一项针对四种人类钩端螺旋体病免疫诊断检测方法的前瞻性评估，包括间接免疫荧光试验（IFA）、显微镜凝集试验（MAT）、钩端螺旋体病快速检测试纸条以及乳胶凝集（LA）试验。从348例出现急性发热但无定位体征的患者中采集了492份血清样本。IFA、MAT、试纸条和LA的敏感性分别为91.9%、76.6%、77.4%和83.1%。这些检测方法的特异性分别为100.0%、100.0%、89.3%和83.5%。尽管IFA显示出最高的总体敏感性和特异性，但在考虑急性期血清时，LA最为敏感（28.7%）。所有三种属特异性抗体检测方法对各种血清群均具有广泛的反应性。MAT最适合参考实验室，在那里可以保存一系列活抗原；IFA适合配备精密设备和专业技术的实验室；试纸条和LA适合缺乏昂贵设备和专业知识的基层实验室。
