Sevcencu C, Rijkhoff N J M, Gregersen H, Sinkjaer T
Center for Sensory-Motor Interaction (SMI), Department of Health Science and Technology, Aalborg University, Denmark.
Neurogastroenterol Motil. 2005 Jun;17(3):376-87. doi: 10.1111/j.1365-2982.2004.00637.x.
This work was performed to study electrically induced contractions in the descending colon of pigs. Contractions were monitored using impedance planimetry and manometry. The luminal pressure, cross-sectional area (CSA), latency and velocity of CSA decrease were compared when using 3 ms, 9, 12, 15 or 30 mA pulses at 10 Hz for 10 s, and 15 mA, 0.03, 0.3 or 3 ms pulses at 10 Hz for 10 s. Stimulation was performed prior and after the application of N(G)-nitro-L-arginine methyl ester (L-NAME) and atropine. In the untreated colon, contraction was always of an 'off' type. A current increase from 9 to 30 mA increased the pressure. An increase of pulse duration from 0.03 to 3 ms shortened the latency, accelerated contraction and increased pressure. By sequential stimulation, contractions were coordinated to propel semi-fluid and solid luminal contents. L-NAME increased the magnitude of CSA decrease. Atropine induced inhibitory effects on contractions elicited by 3 ms pulses and abolished contractions induced by 0.03 and 0.3 ms pulses.
(i) electrical stimulation evokes'off' colon contractions, which can be coordinated to result in propulsion; (ii) the best combination for current and pulse duration to induce propulsive contractions is 15 mA and 3 ms; (iii) nitrergic and cholinergic pathways mediate responses to electrical stimulation.
本研究旨在探究电刺激对猪降结肠收缩的影响。采用阻抗平面测量法和压力测量法监测结肠收缩情况。分别使用频率为10Hz、时长为10s的3ms、9ms、12ms、15ms或30ms的脉冲电流，以及频率为10Hz、时长为10s的15mA、0.03ms、0.3ms或3ms的脉冲电流进行刺激，并在给予N（G）-硝基-L-精氨酸甲酯（L-NAME）和阿托品前后进行刺激。在未处理的结肠中，收缩总是呈“关闭”型。电流从9mA增加到30mA会使压力升高。脉冲持续时间从0.03ms增加到3ms会缩短潜伏期、加速收缩并增加压力。通过连续刺激，收缩可协同推进半流体和固体肠内容物。L-NAME增加了横截面积减小的幅度。阿托品对3ms脉冲引起的收缩有抑制作用，并消除了0.03ms和0.3ms脉冲引起的收缩。
（i）电刺激可引发结肠“关闭”型收缩，且这些收缩可协同导致推进作用；（ii）诱导推进性收缩的电流和脉冲持续时间的最佳组合是15mA和3ms；（iii）一氧化氮能和胆碱能途径介导对电刺激的反应。
