大鼠肝脏再生过程中激活素及激活素受体的表达变化
Activin and activin receptor expression changes in liver regeneration in rat.
作者信息
Takamura Kazuhito, Tsuchida Kunihiro, Miyake Hidenori, Tashiro Seiki, Sugino Hiromu
机构信息
Department of Digestive Surgery, University of Tokushima, Tokushima 770-8503, Japan.
出版信息
J Surg Res. 2005 Jun 1;126(1):3-11. doi: 10.1016/j.jss.2005.01.002.
BACKGROUND
This study aimed to investigate regulatory mechanisms of hepatocyte proliferation by comparing liver regeneration of the remnant lobe after 70% partial hepatectomy (PH) and portal vein branch ligation (PBL) in rat.
METHODS
Expressions of activins betaA, betaC, and betaE and their receptors were investigated after PH and PBL. The proliferating cell nuclear antigen (PCNA) labeling index was used to monitor hepatocyte proliferation.
RESULTS
The PCNA labeling index in the regenerative lobe of PBL rats reached a peak at 48 h, a delay of 24 h compared with the remnant lobe in PH rats. In the postoperative early stage, the expression of activin betaA, betaC, and betaE mRNAs was stronger in PBL than PH. At 72 h the expression of activin receptor type IIA mRNA reached a peak in PH but was significantly lower in PBL.
CONCLUSIONS
Hepatocyte proliferation, and the regulated expression of activins and their receptors, differs during liver regeneration after PH and PBL in the rat. Thus, regulation of activin signaling through receptors is one of the factors determining liver regeneration after PH and PBL.
背景
本研究旨在通过比较大鼠70%肝部分切除术（PH）和门静脉分支结扎术（PBL）后残肝叶的肝再生情况，探讨肝细胞增殖的调控机制。
方法
研究了PH和PBL术后激活素βA、βC和βE及其受体的表达情况。采用增殖细胞核抗原（PCNA）标记指数监测肝细胞增殖。
结果
PBL大鼠再生肝叶中的PCNA标记指数在48小时达到峰值，比PH大鼠残肝叶延迟24小时。术后早期，PBL中激活素βA、βC和βE mRNA的表达比PH更强。在72小时时，IIA型激活素受体mRNA的表达在PH中达到峰值，但在PBL中显著降低。
结论
大鼠PH和PBL后的肝再生过程中，肝细胞增殖以及激活素及其受体的调控表达存在差异。因此，通过受体对激活素信号的调控是决定PH和PBL后肝再生的因素之一。
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