Zhang Xiaoquan, Green Kortney E, Yallampalli Chandra, Dong Yuan Lin
Department of Obstetrics and Gynecology, University of Texas Medical Branch at Galveston, 77555, USA.
Biol Reprod. 2005 Oct;73(4):619-26. doi: 10.1095/biolreprod.105.040436. Epub 2005 May 25.
We have tested the hypothesis that adrenomedullin (ADM), a multifunctional peptide hormone, works as a trophoblast proinvasion factor. Our results showed that ADM receptor components-the mRNA and proteins of calcitonin receptor-like receptor (CALCRL) and receptor activity modifying proteins (RAMPs)-were expressed by human choriocarcinoma JAr cells and first-trimester cytotrophoblast HTR-8/SV neo cells. ADM stimulates both JAr and HTR-8/SV neo cell proliferation. The invasion capabilities of JAr cells and HTR-8/SV neo cells were also enhanced by ADM, and this was associated with increased gelatinolytic activity and reduced plasminogen activator inhibitor-1 mRNA expression (SERPINE1). Our data support the notion that ADM may be involved in the human implantation process via regulating trophoblast proliferation and differentiation.
肾上腺髓质素（ADM），一种多功能肽类激素，作为滋养层促侵袭因子发挥作用。我们的结果显示，ADM受体成分——降钙素受体样受体（CALCRL）的mRNA和蛋白以及受体活性修饰蛋白（RAMP）——在人绒毛膜癌JAr细胞和孕早期细胞滋养层HTR-8/SV neo细胞中表达。ADM刺激JAr细胞和HTR-8/SV neo细胞增殖。ADM还增强了JAr细胞和HTR-8/SV neo细胞的侵袭能力，这与明胶酶活性增加和纤溶酶原激活物抑制剂-1 mRNA表达（SERPINE1）降低有关。我们的数据支持如下观点：ADM可能通过调节滋养层增殖和分化参与人类着床过程。
