IB期宫颈鳞状细胞癌中的细胞凋亡标志物
Markers of apoptosis in stage IB squamous cervical carcinoma.
作者信息
Van de Putte G, Holm R, Lie A K, Baekelandt M, Kristensen G B
机构信息
Department of Gynaecological Oncology, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway.
出版信息
J Clin Pathol. 2005 Jun;58(6):590-4. doi: 10.1136/jcp.2004.021220.
AIMS
To examine the prognostic relevance of the expression of the Bcl-2, Bcl-xL, and Bax proteins in stage IB squamous cervical carcinoma (SCC).
METHODS
In total, 220 patients who underwent radical hysterectomy and bilateral lymphadenectomy at the Norwegian Radium Hospital for stage IB SCC between 1987 and 1993 were studied. Immunohistochemistry using monoclonal antibodies against Bcl-2, Bcl-xL, and Bax was used to examine protein expression. Ten patients who underwent hysterectomy for uterine prolapse served as controls.
RESULTS
Cytoplasmic expression of Bcl-2, Bcl-xL, and Bax was low (< 5% positive cells) in 159 of 220 (73%), 193 of 220 (87%), and 39 of 220 (18%) tumours, respectively, and high (> or = 5% positive cells) in 61 of 220 (27%), 27 of 220 (13%), and 181 of 220 (82%) tumours, respectively. In univariate analysis, all classic clinicopathological parameters but none of the investigated proteins were associated with prognosis. In multivariate analysis, only deep stromal invasion was independently related to survival.
CONCLUSION
Bcl-2, Bcl-xL, and Bax were not independently associated with prognosis in stage IB SCC.
目的
研究Bcl-2、Bcl-xL和Bax蛋白表达在IB期宫颈鳞癌（SCC）中的预后相关性。
方法
对1987年至1993年间在挪威镭医院因IB期SCC接受根治性子宫切除术和双侧淋巴结清扫术的220例患者进行研究。采用抗Bcl-2、Bcl-xL和Bax的单克隆抗体免疫组化法检测蛋白表达。10例行子宫脱垂子宫切除术的患者作为对照。
结果
220例肿瘤中，分别有159例（73%）、193例（87%）和39例（18%）肿瘤的Bcl-2、Bcl-xL和Bax的细胞质表达低（阳性细胞<5%），分别有61例（27%）、27例（13%）和181例（82%）肿瘤的表达高（阳性细胞≥5%）。单因素分析中，除所研究的蛋白外，所有经典临床病理参数均与预后相关。多因素分析中，仅深部间质浸润与生存独立相关。
结论
在IB期SCC中，Bcl-2、Bcl-xL和Bax与预后无独立相关性。
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