利用酿酒酵母的靶标特异性、超敏菌株来确定抗真菌化合物的作用模式。
Utilization of target-specific, hypersensitive strains of Saccharomyces cerevisiae to determine the mode of action of antifungal compounds.
作者信息
Buurman Ed T, Andrews Beth, Blodgett April E, Chavda Jini S, Schnell Norbert F
机构信息
Department of Microbiology, AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, MA 02451, USA.
出版信息
Antimicrob Agents Chemother. 2005 Jun;49(6):2558-60. doi: 10.1128/AAC.49.6.2558-2560.2005.
Abstract
Target-specific hypersusceptible strains of Saccharomyces cerevisiae were used to screen antifungal compounds. Two novel Erg7p inhibitors were identified, providing proof of principle of the approach taken. However, observed hypersensitivities to antifungals acting via other targets imply that use of this tool to identify the mode of action requires significant deconvolution.
摘要
利用酿酒酵母的靶向特异性超敏感菌株筛选抗真菌化合物。鉴定出两种新型的Erg7p抑制剂，证明了所采用方法的原理。然而，观察到的对通过其他靶点起作用的抗真菌药物的超敏感性表明，使用该工具来确定作用模式需要进行大量的解析工作。
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