大鼠CCL9（MIP-1γ）的分子克隆与特性分析，其为小鼠CCL9的直系同源物。
Molecular cloning and characterization of rat CCL9 (MIP-1gamma), the ortholog of mouse CCL9.
作者信息
Yang Meiheng, Odgren Paul R
机构信息
Department of Cell Biology, 7th Floor, University of Massachusetts Medical School, 55 Lake Avenue, North Worcester, MA 01655, USA.
出版信息
Cytokine. 2005 Jul 21;31(2):94-102. doi: 10.1016/j.cyto.2005.04.001.
We identified an EST sequence that was up-regulated during osteoclast formation in the rat. Investigating further, we cloned the cDNA from rat long bone and found it to be highly homologous to the mouse CC chemokine, CCL9, both at the nucleotide and amino acid levels. The rat CCL9 amino acid sequence is 74% identical to the mouse sequence, with an additional 11% similar amino acids. Recombinant rat CCL9 was used in chemotaxis assays of rat bone marrow cells and it was found to have a strong and dose-dependent effect. In addition, CCL9 mRNA was very highly up-regulated during osteoclast differentiation of rat bone marrow-derived mononuclear cells, increasing by over 100-fold when stimulated by colony stimulating factor-1 and the TNF superfamily member, RANKL. Together, these results establish that, like the mouse, the rat also uses CCL9 to promote the complex process of osteoclast formation.
我们鉴定出一个在大鼠破骨细胞形成过程中上调的EST序列。进一步研究发现，我们从大鼠长骨中克隆出cDNA，发现其在核苷酸和氨基酸水平上均与小鼠CC趋化因子CCL9高度同源。大鼠CCL9氨基酸序列与小鼠序列的同源性为74%，另有11%的氨基酸相似。重组大鼠CCL9用于大鼠骨髓细胞的趋化性分析，发现其具有强烈的剂量依赖性效应。此外，在大鼠骨髓来源的单核细胞破骨细胞分化过程中，CCL9 mRNA高度上调，在集落刺激因子-1和TNF超家族成员RANKL刺激下增加超过100倍。这些结果共同表明，与小鼠一样，大鼠也利用CCL9来促进破骨细胞形成这一复杂过程。
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