Ramírez-Ramírez Alicia, Sánchez-Tejeda Gustavo, Méndez-Galván Jorge, Unnasch Thomas R, Monroy-Ostria Amalia
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, Col. Casco de Santo Tomás, C.P. 11340, México D.F., Mexico.
Infect Genet Evol. 2006 May;6(3):171-6. doi: 10.1016/j.meegid.2005.03.001.
DNA from Onchocerca volvulus from Oaxaca and Chiapas, Mexico were used as templates to amplify members of the O-150 Onchocerca specific repeat sequence family. The resulting PCR amplicons all hybridized with OVS2, an oligonucleotide that has been previously shown to recognize amplicons derived from O. volvulus with 100% sensitivity. However, when PCR products amplified from the O. volvulus specific plasmid pOVS134 were used as a probe, most samples did not hybridize. Similarly, when PCR products amplified from DNA isolated from adult O. volvulus from Oaxaca were used as a probe, amplicons from adult worms from both Oaxaca and Chiapas were recognized, but PCR products from infected black flies from Chiapas were not recognized. Amplicons derived from an adult worm from Chiapas hybridized with PCR products produced from adult parasites from both Oaxaca and Chiapas and to PCR products derived from the DNA of infected black flies from Chiapas. These data, when taken together, suggest that differences exist among the repeat sequence populations of parasites from Oaxaca and Chiapas in Mexico, suggesting that the O-150 repeat sequence family may be a useful tool for biogeographic studies of O. volvulus in the Americas.
来自墨西哥瓦哈卡州和恰帕斯州的盘尾丝虫的DNA被用作模板，以扩增O-150盘尾丝虫特异性重复序列家族的成员。所得的PCR扩增产物均与OVS2杂交，OVS2是一种寡核苷酸，先前已证明它能以100%的灵敏度识别源自盘尾丝虫的扩增产物。然而，当将从盘尾丝虫特异性质粒pOVS134扩增的PCR产物用作探针时，大多数样品未杂交。同样，当将从瓦哈卡州成年盘尾丝虫分离的DNA扩增的PCR产物用作探针时，瓦哈卡州和恰帕斯州成年虫体的扩增产物均被识别，但恰帕斯州受感染黑蝇的PCR产物未被识别。源自恰帕斯州一条成年虫体的扩增产物与瓦哈卡州和恰帕斯州成年寄生虫产生的PCR产物以及恰帕斯州受感染黑蝇DNA衍生的PCR产物杂交。综合这些数据表明，墨西哥瓦哈卡州和恰帕斯州寄生虫的重复序列群体之间存在差异，这表明O-150重复序列家族可能是美洲盘尾丝虫生物地理学研究的有用工具。
