Spelbrink Robin E J, Kolkman Annemieke, Slijper Monique, Killian J Antoinette, de Kruijff Ben
Department Biochemistry of Membranes, Institute of Biomembranes and Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
J Biol Chem. 2005 Aug 5;280(31):28742-8. doi: 10.1074/jbc.M501617200. Epub 2005 May 25.
In this study we present a new technology to detect stable oligomeric protein complexes in membranes. The technology is based on the ability of small membrane-active alcohols to dissociate the highly stable homotetrameric potassium channel KcsA. It is shown via a proteomics approach, using diagonal electrophoresis and nano-flow liquid chromatography coupled to tandem mass spectrometry, that a large number of both integral and peripheral Escherichia coli inner membrane proteins are part of stable oligomeric complexes that can be dissociated by small alcohols. This study gives insight into the composition and stability of these complexes.
在本研究中，我们提出了一种检测膜中稳定寡聚蛋白复合物的新技术。该技术基于小分子膜活性醇解离高度稳定的同四聚体钾通道KcsA的能力。通过蛋白质组学方法，利用对角线电泳和与串联质谱联用的纳流液相色谱法表明，大量的大肠杆菌内膜整合蛋白和外周蛋白都是稳定寡聚复合物的一部分，这些复合物可被小分子醇解离。本研究深入了解了这些复合物的组成和稳定性。
