• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Mutations in the NS1 protein of swine influenza virus impair anti-interferon activity and confer attenuation in pigs.猪流感病毒NS1蛋白的突变会损害抗干扰素活性并导致猪的病毒减毒。
J Virol. 2005 Jun;79(12):7535-43. doi: 10.1128/JVI.79.12.7535-7543.2005.
2
A single amino acid substitution in the West Nile virus nonstructural protein NS2A disables its ability to inhibit alpha/beta interferon induction and attenuates virus virulence in mice.西尼罗河病毒非结构蛋白NS2A中的单个氨基酸替换使其丧失抑制α/β干扰素诱导的能力，并减弱了病毒在小鼠中的毒力。
J Virol. 2006 Mar;80(5):2396-404. doi: 10.1128/JVI.80.5.2396-2404.2006.
3
The N- and C-terminal domains of the NS1 protein of influenza B virus can independently inhibit IRF-3 and beta interferon promoter activation.乙型流感病毒NS1蛋白的N端和C端结构域可独立抑制IRF-3和β干扰素启动子的激活。
J Virol. 2004 Nov;78(21):11574-82. doi: 10.1128/JVI.78.21.11574-11582.2004.
4
Effect on virulence and pathogenicity of H5N1 influenza A virus through truncations of NS1 eIF4GI binding domain.通过截短 NS1 与 eIF4GI 结合结构域对 H5N1 流感病毒毒力和致病性的影响。
J Infect Dis. 2010 Nov 1;202(9):1338-46. doi: 10.1086/656536.
5
The NS1 gene of H5N1 influenza viruses circumvents the host anti-viral cytokine responses.H5N1流感病毒的NS1基因规避宿主抗病毒细胞因子反应。
Virus Res. 2004 Jul;103(1-2):107-13. doi: 10.1016/j.virusres.2004.02.022.
6
Variation in the ability of human influenza A viruses to induce and inhibit the IFN-beta pathway.甲型人流感病毒诱导和抑制IFN-β途径能力的变异
Virology. 2006 Mar 30;347(1):52-64. doi: 10.1016/j.virol.2005.11.024. Epub 2005 Dec 27.
7
Rescue of influenza virus expressing GFP from the NS1 reading frame.从NS1阅读框拯救表达绿色荧光蛋白的流感病毒。
Virology. 2004 Jun 20;324(1):67-73. doi: 10.1016/j.virol.2004.03.035.
8
Characterization and evaluation of avian influenza NS1 mutant virus as a potential live and killed DIVA (differentiating between infected and vaccinated animals) vaccine for chickens.鉴定和评估禽流感 NS1 突变病毒作为一种潜在的活疫苗和死疫苗（区分感染动物和免疫动物）在鸡中的应用。
Vaccine. 2010 Mar 11;28(12):2388-96. doi: 10.1016/j.vaccine.2009.12.074. Epub 2010 Jan 10.
9
Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach.表达改变的NS1蛋白的甲型和乙型流感病毒：一种疫苗策略。
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4309-14. doi: 10.1073/pnas.070525997.
10
[Study progress of NS1 protein of influenza A virus].甲型流感病毒NS1蛋白的研究进展
Wei Sheng Wu Xue Bao. 2007 Aug;47(4):729-33.

引用本文的文献

1
Synthetic biology-inspired development of live attenuated influenza vaccines.受合成生物学启发的减毒活流感疫苗研发。
NPJ Vaccines. 2025 Aug 27;10(1):204. doi: 10.1038/s41541-025-01255-1.
2
A single-dose intranasal immunization with a novel bat influenza A virus-vectored MERS vaccine provides effective protection against lethal MERS-CoV challenge.用一种新型甲型蝙蝠流感病毒载体中东呼吸综合征疫苗进行单剂量鼻内免疫可有效抵御致死性中东呼吸综合征冠状病毒攻击。
mBio. 2025 Jun 30:e0110725. doi: 10.1128/mbio.01107-25.
3
The applications of live attenuated influenza a virus with modified NS1 gene.具有修饰NS1基因的甲型流感病毒减毒活疫苗的应用。
Mol Ther Nucleic Acids. 2025 Feb 1;36(1):102471. doi: 10.1016/j.omtn.2025.102471. eCollection 2025 Mar 11.
4
Cellular NS1-BP protein interacts with the mRNA export receptor NXF1 to mediate nuclear export of influenza virus M mRNAs.细胞NS1-BP蛋白与mRNA输出受体NXF1相互作用，介导流感病毒M mRNA的核输出。
J Biol Chem. 2024 Nov;300(11):107871. doi: 10.1016/j.jbc.2024.107871. Epub 2024 Oct 9.
5
Adjuvant Use of the Invariant-Natural-Killer-T-Cell Agonist α-Galactosylceramide Leads to Vaccine-Associated Enhanced Respiratory Disease in Influenza-Vaccinated Pigs.不变自然杀伤T细胞激动剂α-半乳糖神经酰胺的辅助使用导致流感疫苗接种猪出现与疫苗相关的呼吸道疾病加重。
Vaccines (Basel). 2024 Sep 18;12(9):1068. doi: 10.3390/vaccines12091068.
6
The C-terminal amino acid motifs of NS1 protein affect the replication and virulence of naturally NS-truncated H1N1 canine influenza virus.NS1 蛋白的 C 末端氨基酸基序影响自然发生的 NS 截断 H1N1 犬流感病毒的复制和毒力。
Emerg Microbes Infect. 2024 Dec;13(1):2400546. doi: 10.1080/22221751.2024.2400546. Epub 2024 Sep 13.
7
Swine influenza A virus: challenges and novel vaccine strategies.猪流感 A 病毒：挑战与新型疫苗策略。
Front Cell Infect Microbiol. 2024 Apr 3;14:1336013. doi: 10.3389/fcimb.2024.1336013. eCollection 2024.
8
A clinically attenuated double-mutant of porcine reproductive and respiratory syndrome virus-2 that does not prompt overexpression of proinflammatory cytokines during co-infection with a secondary pathogen.一种临床减毒的猪繁殖与呼吸综合征病毒-2 双突变株，在与次级病原体共感染时不会引发促炎细胞因子的过度表达。
PLoS Pathog. 2024 Mar 28;20(3):e1012128. doi: 10.1371/journal.ppat.1012128. eCollection 2024 Mar.
9
Antiviral responses versus virus-induced cellular shutoff: a game of thrones between influenza A virus NS1 and SARS-CoV-2 Nsp1.抗病毒反应与病毒诱导的细胞关闭：甲型流感病毒NS1与严重急性呼吸综合征冠状病毒2 Nsp1之间的权力游戏
Front Cell Infect Microbiol. 2024 Feb 5;14:1357866. doi: 10.3389/fcimb.2024.1357866. eCollection 2024.
10
Pathogenesis and Transmission Assessment of 3 Swine-Origin Influenza A(H3N2) Viruses With Zoonotic Risk to Humans Isolated in the United States, 2017-2020.2017-2020 年美国分离的具有人类感染风险的 3 种猪源甲型流感病毒（H3N2）的发病机制和传播评估。
J Infect Dis. 2024 Apr 12;229(4):1107-1111. doi: 10.1093/infdis/jiad359.

本文引用的文献

1
The NS1 gene of H5N1 influenza viruses circumvents the host anti-viral cytokine responses.H5N1流感病毒的NS1基因规避宿主抗病毒细胞因子反应。
Virus Res. 2004 Jul;103(1-2):107-13. doi: 10.1016/j.virusres.2004.02.022.
2
Multiple lineages of antigenically and genetically diverse influenza A virus co-circulate in the United States swine population.多种抗原性和基因不同的甲型流感病毒谱系在美国猪群中共同传播。
Virus Res. 2004 Jul;103(1-2):67-73. doi: 10.1016/j.virusres.2004.02.015.
3
Defective RNA replication and late gene expression in temperature-sensitive influenza viruses expressing deleted forms of the NS1 protein.表达NS1蛋白缺失形式的温度敏感型流感病毒中的RNA复制缺陷和晚期基因表达
J Virol. 2004 Apr;78(8):3880-8. doi: 10.1128/jvi.78.8.3880-3888.2004.
4
Human influenza virus NS1 protein enhances viral pathogenicity and acts as an RNA silencing suppressor in plants.人类流感病毒NS1蛋白可增强病毒致病性，并在植物中作为一种RNA沉默抑制因子发挥作用。
J Gen Virol. 2004 Apr;85(Pt 4):993-999. doi: 10.1099/vir.0.19735-0.
5
The influenza A virus NS1 protein binds small interfering RNAs and suppresses RNA silencing in plants.甲型流感病毒NS1蛋白可结合小干扰RNA并抑制植物中的RNA沉默。
J Gen Virol. 2004 Apr;85(Pt 4):983-991. doi: 10.1099/vir.0.19734-0.
6
Biophysical characterization of the complex between double-stranded RNA and the N-terminal domain of the NS1 protein from influenza A virus: evidence for a novel RNA-binding mode.甲型流感病毒NS1蛋白N端结构域与双链RNA复合物的生物物理特性：一种新型RNA结合模式的证据
Biochemistry. 2004 Feb 24;43(7):1950-62. doi: 10.1021/bi030176o.
7
The influenza B virus nonstructural NS1 protein is essential for efficient viral growth and antagonizes beta interferon induction.乙型流感病毒非结构蛋白NS1对于病毒的高效生长至关重要，并可拮抗β干扰素的诱导。
J Virol. 2004 Feb;78(4):1865-72. doi: 10.1128/jvi.78.4.1865-1872.2004.
8
Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing.流感病毒和痘苗病毒的干扰素拮抗剂蛋白是RNA沉默的抑制剂。
Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1350-5. doi: 10.1073/pnas.0308308100. Epub 2004 Jan 26.
9
PABP1 and eIF4GI associate with influenza virus NS1 protein in viral mRNA translation initiation complexes.PABP1和eIF4GI在病毒mRNA翻译起始复合物中与流感病毒NS1蛋白相关联。
J Gen Virol. 2003 Dec;84(Pt 12):3263-3274. doi: 10.1099/vir.0.19487-0.
10
A recombinant influenza A virus expressing an RNA-binding-defective NS1 protein induces high levels of beta interferon and is attenuated in mice.表达RNA结合缺陷型NS1蛋白的重组甲型流感病毒可诱导高水平的β干扰素，且在小鼠中减毒。
J Virol. 2003 Dec;77(24):13257-66. doi: 10.1128/jvi.77.24.13257-13266.2003.

猪流感病毒NS1蛋白的突变会损害抗干扰素活性并导致猪的病毒减毒。

Mutations in the NS1 protein of swine influenza virus impair anti-interferon activity and confer attenuation in pigs.

作者信息

Solórzano Alicia, Webby Richard J, Lager Kelly M, Janke Bruce H, García-Sastre Adolfo, Richt Jürgen A

机构信息

National Animal Disease Center, Ames, IA 50010, USA.

出版信息

J Virol. 2005 Jun;79(12):7535-43. doi: 10.1128/JVI.79.12.7535-7543.2005.

DOI:10.1128/JVI.79.12.7535-7543.2005
PMID:15919908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1143661/
Abstract

It has been shown previously that the nonstructural protein NS1 of influenza virus is an alpha/beta interferon (IFN-alpha/beta) antagonist, both in vitro and in experimental animal model systems. However, evidence of this function in a natural host has not yet been obtained. Here we investigated the role of the NS1 protein in the virulence of a swine influenza virus (SIV) isolate in pigs by using reverse genetics. The virulent wild-type A/Swine/Texas/4199-2/98 (TX/98) virus and various mutants encoding carboxy-truncated NS1 proteins were rescued. Growth properties of TX/98 viruses with mutated NS1, induction of IFN in tissue culture, and virulence-attenuation in pigs were analyzed and compared to those of the recombinant wild-type TX/98 virus. Our results indicate that deletions in the NS1 protein decrease the ability of the TX/98 virus to prevent IFN-alpha/beta synthesis in pig cells. Moreover, all NS1 mutant viruses were attenuated in pigs, and this correlated with the amount of IFN-alpha/beta induced in vitro. These data suggest that the NS1 protein of SIV is a virulence factor. Due to their attenuation, NS1-mutated swine influenza viruses might have a great potential as live attenuated vaccine candidates against SIV infections of pigs.

摘要

先前研究表明，在体外实验和实验动物模型系统中，流感病毒的非结构蛋白NS1是α/β干扰素（IFN-α/β）拮抗剂。然而，尚未获得该功能在自然宿主中的证据。在此，我们通过反向遗传学研究了NS1蛋白在猪流感病毒（SIV）分离株对猪的毒力中的作用。拯救了强毒野生型A/猪/得克萨斯/4199-2/98（TX/98）病毒及各种编码羧基末端截短NS1蛋白的突变体。分析并比较了具有突变NS1的TX/98病毒的生长特性、在组织培养中IFN的诱导情况以及在猪体内的毒力减弱情况，并与重组野生型TX/98病毒进行对比。我们的结果表明，NS1蛋白缺失会降低TX/98病毒阻止猪细胞中IFN-α/β合成的能力。此外，所有NS1突变病毒在猪体内毒力均减弱，且这与体外诱导产生的IFN-α/β量相关。这些数据表明，SIV的NS1蛋白是一种毒力因子。由于其毒力减弱，NS1突变的猪流感病毒作为预防猪SIV感染的减毒活疫苗候选株可能具有巨大潜力。