乙型肝炎病毒大表面抗原基质区与跨膜区之间最小距离的测定
Determination of the minimal distance between the matrix and transmembrane domains of the large hepatitis B virus envelope protein.
作者信息
Kluge Britta, Schläger Michaela, Pairan Alexander, Bruss Volker
机构信息
University of Göttingen, Department of Virology, Kreuzbergring 57, D-37075 Göttingen, Germany.
出版信息
J Virol. 2005 Jun;79(12):7918-21. doi: 10.1128/JVI.79.12.7918-7921.2005.
Abstract
The cytosolic matrix domain (MD) located between amino acids (aa) 103 and 124 of the large hepatitis B virus envelope protein L is essential for virion formation. We reduced the distance between MD and the transmembrane domain (TD; aa 254 to 272) by deletions starting at aa 132. Six mutants with deletions of up to aa 234 were wild type, and four mutants with slightly larger deletions were blocked with respect to virion morphogenesis. Thus, the minimal distance between MD and TD was around 26 aa. This spacer might be required by MD to reach contact sites on the capsid.
摘要
乙型肝炎病毒大表面蛋白L中位于氨基酸（aa）103和124之间的胞质基质结构域（MD）对于病毒粒子的形成至关重要。我们从氨基酸132开始进行缺失，以缩短MD与跨膜结构域（TD；氨基酸254至272）之间的距离。六个缺失至氨基酸234的突变体为野生型，而四个缺失稍大的突变体在病毒粒子形态发生方面受阻。因此，MD与TD之间的最小距离约为26个氨基酸。这个间隔区可能是MD到达衣壳上接触位点所必需的。
相似文献
1
Determination of the minimal distance between the matrix and transmembrane domains of the large hepatitis B virus envelope protein.乙型肝炎病毒大表面抗原基质区与跨膜区之间最小距离的测定
J Virol. 2005 Jun;79(12):7918-21. doi: 10.1128/JVI.79.12.7918-7921.2005.
2
Effect of mutations in the small envelope protein of hepatitis B virus on assembly and secretion of hepatitis delta virus.乙型肝炎病毒小包膜蛋白突变对丁型肝炎病毒组装和分泌的影响。
Virology. 1998 Nov 10;251(1):176-86. doi: 10.1006/viro.1998.9391.
3
Envelopment of the hepatitis B virus nucleocapsid.乙型肝炎病毒核衣壳的包被
Virus Res. 2004 Dec;106(2):199-209. doi: 10.1016/j.virusres.2004.08.016.
4
Analysis of the cytosolic domains of the hepatitis B virus envelope proteins for their function in viral particle assembly and infectivity.分析乙型肝炎病毒包膜蛋白的胞质结构域在病毒颗粒组装和感染性中的功能。
J Virol. 2006 Dec;80(24):11935-45. doi: 10.1128/JVI.00621-06. Epub 2006 Oct 4.
5
Mutational profiling of the variability of individual amino acid positions in the hepatitis B virus matrix domain.乙型肝炎病毒基质结构域中单个氨基酸位置变异性的突变分析。
Virology. 2014 Jun;458-459:183-9. doi: 10.1016/j.virol.2014.04.030. Epub 2014 May 14.
6
A short linear sequence in the pre-S domain of the large hepatitis B virus envelope protein required for virion formation.乙肝病毒大表面抗原前S区中一段形成病毒体所需的短线性序列。
J Virol. 1997 Dec;71(12):9350-7. doi: 10.1128/JVI.71.12.9350-9357.1997.
7
Hepatitis B virus assembly is sensitive to changes in the cytosolic S loop of the envelope proteins.乙型肝炎病毒组装对包膜蛋白胞质 S 环的变化敏感。
Virology. 2000 May 10;270(2):358-67. doi: 10.1006/viro.2000.0268.
8
Two distinct segments of the hepatitis B virus surface antigen contribute synergistically to its association with the viral core particles.
J Mol Biol. 1999 Feb 26;286(3):797-808. doi: 10.1006/jmbi.1998.2525.
9
Chimeric hepatitis B and C viruses envelope proteins can form subviral particles: implications for the design of new vaccine strategies.嵌合型乙肝和丙肝病毒包膜蛋白可形成亚病毒颗粒：对新型疫苗策略设计的启示
N Biotechnol. 2009 Apr;25(4):226-34. doi: 10.1016/j.nbt.2009.01.001. Epub 2009 Jan 21.
10
Single and multiple deletions in the transmembrane domain of the Sindbis virus E2 glycoprotein identify a region critical for normal virus growth.辛德毕斯病毒E2糖蛋白跨膜结构域中的单缺失和多缺失鉴定出对正常病毒生长至关重要的区域。
Virology. 2006 Mar 30;347(1):199-207. doi: 10.1016/j.virol.2005.11.029. Epub 2006 Jan 4.
引用本文的文献
1
An E. coli-produced single-chain variable fragment (scFv) targeting hepatitis B virus surface protein potently inhibited virion secretion.大肠杆菌产生的针对乙型肝炎病毒表面蛋白的单链可变片段（scFv）可有效抑制病毒粒子的分泌。
Antiviral Res. 2019 Feb;162:118-129. doi: 10.1016/j.antiviral.2018.12.019. Epub 2018 Dec 30.
2
Common and Distinct Capsid and Surface Protein Requirements for Secretion of Complete and Genome-Free Hepatitis B Virions.完整和无基因组乙型肝炎病毒粒子分泌的常见和独特衣壳及表面蛋白需求。
J Virol. 2018 Jun 29;92(14). doi: 10.1128/JVI.00272-18. Print 2018 Jul 15.
3
An Aptamer against the Matrix Binding Domain on the Hepatitis B Virus Capsid Impairs Virion Formation.一种针对乙肝病毒衣壳上基质结合域的适配体可损害病毒粒子的形成。
J Virol. 2015 Sep;89(18):9281-7. doi: 10.1128/JVI.00466-15. Epub 2015 Jul 1.
4
Host factors involved in hepatitis B virus maturation, assembly, and egress.参与乙型肝炎病毒成熟、组装和出芽的宿主因素。
Med Microbiol Immunol. 2012 Nov;201(4):449-61. doi: 10.1007/s00430-012-0267-9. Epub 2012 Sep 11.
5
The pre-s2 domain of the hepatitis B virus is dispensable for infectivity but serves a spacer function for L-protein-connected virus assembly.乙型肝炎病毒的前 S2 结构域对于感染性是可有可无的，但对于 L 蛋白连接的病毒组装起到了间隔功能。
J Virol. 2010 Apr;84(8):3879-88. doi: 10.1128/JVI.02528-09. Epub 2010 Feb 3.
6
Functional surfaces of the hepatitis B virus capsid.乙肝病毒衣壳的功能表面。
J Virol. 2009 Nov;83(22):11616-23. doi: 10.1128/JVI.01178-09. Epub 2009 Aug 26.
7
Hepatitis B virus morphogenesis.乙型肝炎病毒形态发生
World J Gastroenterol. 2007 Jan 7;13(1):65-73. doi: 10.3748/wjg.v13.i1.65.
8
Analysis of the cytosolic domains of the hepatitis B virus envelope proteins for their function in viral particle assembly and infectivity.分析乙型肝炎病毒包膜蛋白的胞质结构域在病毒颗粒组装和感染性中的功能。
J Virol. 2006 Dec;80(24):11935-45. doi: 10.1128/JVI.00621-06. Epub 2006 Oct 4.
本文引用的文献
1
Envelopment of the hepatitis B virus nucleocapsid.乙型肝炎病毒核衣壳的包被
Virus Res. 2004 Dec;106(2):199-209. doi: 10.1016/j.virusres.2004.08.016.
2
Mapping of amino acid side chains on the surface of hepatitis B virus capsids required for envelopment and virion formation.乙肝病毒衣壳表面上对于包膜化和病毒体形成所必需的氨基酸侧链的定位
J Virol. 2003 Jan;77(1):416-22. doi: 10.1128/jvi.77.1.416-422.2003.
3
Influence of a putative intermolecular interaction between core and the pre-S1 domain of the large envelope protein on hepatitis B virus secretion.核心蛋白与大包膜蛋白前S1结构域之间假定的分子间相互作用对乙型肝炎病毒分泌的影响。
J Virol. 2002 Jul;76(13):6510-7. doi: 10.1128/jvi.76.13.6510-6517.2002.
4
Hepatitis B virus assembly is sensitive to changes in the cytosolic S loop of the envelope proteins.乙型肝炎病毒组装对包膜蛋白胞质 S 环的变化敏感。
Virology. 2000 May 10;270(2):358-67. doi: 10.1006/viro.2000.0268.
5
The crystal structure of the human hepatitis B virus capsid.人类乙型肝炎病毒衣壳的晶体结构。
Mol Cell. 1999 Jun;3(6):771-80. doi: 10.1016/s1097-2765(01)80009-5.
6
Two distinct segments of the hepatitis B virus surface antigen contribute synergistically to its association with the viral core particles.
J Mol Biol. 1999 Feb 26;286(3):797-808. doi: 10.1006/jmbi.1998.2525.
7
Infection process of the hepatitis B virus depends on the presence of a defined sequence in the pre-S1 domain.乙型肝炎病毒的感染过程取决于前S1结构域中特定序列的存在。
J Virol. 1999 Mar;73(3):2052-7. doi: 10.1128/JVI.73.3.2052-2057.1999.
8
Role of the pre-S2 domain of the large envelope protein in hepatitis B virus assembly and infectivity.乙肝病毒大包膜蛋白前S2结构域在病毒装配及感染性中的作用
J Virol. 1998 Jul;72(7):5573-8. doi: 10.1128/JVI.72.7.5573-5578.1998.
9
Dual topology of the large envelope protein of duck hepatitis B virus: determinants preventing pre-S translocation and glycosylation.鸭乙型肝炎病毒大包膜蛋白的双重拓扑结构：阻止前S区易位和糖基化的决定因素
J Virol. 1997 Dec;71(12):9434-41. doi: 10.1128/JVI.71.12.9434-9441.1997.
10
A short linear sequence in the pre-S domain of the large hepatitis B virus envelope protein required for virion formation.乙肝病毒大表面抗原前S区中一段形成病毒体所需的短线性序列。
J Virol. 1997 Dec;71(12):9350-7. doi: 10.1128/JVI.71.12.9350-9357.1997.
Zotero 插件