Kluge Britta, Schläger Michaela, Pairan Alexander, Bruss Volker
University of Göttingen, Department of Virology, Kreuzbergring 57, D-37075 Göttingen, Germany.
J Virol. 2005 Jun;79(12):7918-21. doi: 10.1128/JVI.79.12.7918-7921.2005.
The cytosolic matrix domain (MD) located between amino acids (aa) 103 and 124 of the large hepatitis B virus envelope protein L is essential for virion formation. We reduced the distance between MD and the transmembrane domain (TD; aa 254 to 272) by deletions starting at aa 132. Six mutants with deletions of up to aa 234 were wild type, and four mutants with slightly larger deletions were blocked with respect to virion morphogenesis. Thus, the minimal distance between MD and TD was around 26 aa. This spacer might be required by MD to reach contact sites on the capsid.
乙型肝炎病毒大表面蛋白L中位于氨基酸（aa）103和124之间的胞质基质结构域（MD）对于病毒粒子的形成至关重要。我们从氨基酸132开始进行缺失，以缩短MD与跨膜结构域（TD；氨基酸254至272）之间的距离。六个缺失至氨基酸234的突变体为野生型，而四个缺失稍大的突变体在病毒粒子形态发生方面受阻。因此，MD与TD之间的最小距离约为26个氨基酸。这个间隔区可能是MD到达衣壳上接触位点所必需的。
