鸡艾美耳球虫刺激的肠道上皮内淋巴细胞的表达序列标签分析
Expressed sequence tag analysis of Eimeria-stimulated intestinal intraepithelial lymphocytes in chickens.
作者信息
Min Wongi, Lillehoj Hyun S, Ashwell Christopher M, van Tassell Curtis P, Dalloul Rami A, Matukumalli Lakshmi K, Han Jae Y, Lillehoj Erik P
机构信息
Animal Parasitic Diseases Laboratory, Animal & Natural Resources Institute, US Dept. of Agriculture, Beltsville, MD 20705, USA.
出版信息
Mol Biotechnol. 2005 Jun;30(2):143-50. doi: 10.1385/MB:30:2:143.
Intraepithelial lymphocytes (IELs) play a critical role in protective immune response to intestinal pathogens such as Eimeria, the etiologic agent of avian coccidiosis. A list of genes expressed by intestinal IELs of Eimeria-infected chickens was compiled using the expressed sequence tag (EST) strategy. The 14,409 ESTs consisted of 1851 clusters and 7595 singletons, which revealed 9446 unique genes in the data set. Comparison of the sequence data with chicken DNA sequences in GenBank identified 125 novel clones. This EST library will provide a valuable resource for profiling global gene expression in normal and pathogen-infected chickens and identifying additional unique immune-related genes.
上皮内淋巴细胞（IELs）在对肠道病原体（如引起禽球虫病的艾美耳球虫）的保护性免疫反应中发挥关键作用。利用表达序列标签（EST）策略编制了一份艾美耳球虫感染鸡的肠道IELs所表达基因的清单。这14409个EST由1851个聚类和7595个单拷贝序列组成，在数据集中揭示了9446个独特基因。将序列数据与GenBank中的鸡DNA序列进行比较，鉴定出125个新克隆。这个EST文库将为分析正常和病原体感染鸡的全球基因表达以及鉴定其他独特的免疫相关基因提供宝贵资源。
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