AccuLevel与TDx的比较：抗癫痫药物现场监测评估
Comparison of AccuLevel and TDx: evaluation of on-site monitoring of antiepileptic drugs.
作者信息
Nielsen I M, Gram L, Dam M
机构信息
University Clinic of Neurology, Hvidovre Hospital, Denmark.
出版信息
Epilepsia. 1992 May-Jun;33(3):558-63. doi: 10.1111/j.1528-1157.1992.tb01710.x.
Therapeutic drug monitoring, an important aid in antiepileptic drug (AED) therapy, has a lag time before results are obtained from clinical laboratories. The AccuLevel test is an enzyme immunochromatographic method for quantitative measurement of AEDs including phenobarbital (PB), phenytoin (PHT), and carbamazepine (CBZ), with results available within 20 min. A comparison between AccuLevel and TDx, (fluorescence polarization immunoassay) was conducted in 233 paired blood samples from patients with AED therapy, including 12 Eskimo children receiving treatment in Greenland. Forty-five blood samples were analyzed for PB, 80 for PHT, and 108 for CBZ. Linear regression analysis showed good agreement between the two methods (r = 0.951, 0.958, and 0.945, respectively). The test is easy to perform, but care must be taken to follow the correct procedure, or inaccuracies will result. That happened in some of our results. A reduction in lag time, using on-site drug monitoring, was demonstrated. The AccuLevel is a rapid, accurate, and convenient method for use in AED monitoring.
治疗药物监测是抗癫痫药物（AED）治疗中的一项重要辅助手段，但从临床实验室获得结果存在滞后时间。AccuLevel检测是一种酶免疫色谱法，用于定量测定包括苯巴比妥（PB）、苯妥英（PHT）和卡马西平（CBZ）在内的抗癫痫药物，20分钟内即可获得结果。对233份接受AED治疗患者的配对血样进行了AccuLevel检测与TDx（荧光偏振免疫测定法）的比较，其中包括12名在格陵兰接受治疗的爱斯基摩儿童。对45份血样进行了PB分析，80份进行了PHT分析，108份进行了CBZ分析。线性回归分析表明两种方法之间具有良好的一致性（r分别为0.951、0.958和0.945）。该检测操作简便，但必须注意遵循正确的程序，否则会导致结果不准确。我们的一些结果就出现了这种情况。结果表明，采用现场药物监测可缩短滞后时间。AccuLevel是一种用于AED监测的快速、准确且方便的方法。
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